TABLE 1.

Description of EVs studieda

Clinical samplebGenBank accession no.Neutrali-zation sero-typescPhylogenetic genotypeBoot-strap valueClustal homology test index (%)dGenotype determined by clustal homology testePileup homology test index(%)eGenotype determined by pileup homol-ogy testeHighest score NW-GCG similarity test index (%)fSecond highest score NW-GCG similarity test index (%)gGenotype determined by NW-GCG similarity teste
TX92-1647AF081634CAV14CAV1410087.7CAV1487.5CAV1487.263.5CAV14
GA95-2095AF081613CAV16CAV168678.9CAV1678.9CAV1678.170.1CAV16
PA94-5753AF081628CAV16CAV168678.9CAV1678.9CAV1678.169.3CAV16
TX95-2147AF081635CAV16CAV168677.7CAV1677.7CAV1676.871.2CAV16
PA89-9262AF152288CAV16CAV168678.9CAV1678.9CAV1678.170.2CAV16
TAI84-5839AF152293CAV16CAV168679.1CAV1679.1CAV1678.370.2CAV16
707F99AF290902CAV16CAV168681CAV1681.5CAV1680.864.9CAV16
TN88-8321AF152294CAV17CAV179979.5CAV1779.6CAV1779.370.2CAV17
MOR83-6282AF152273CAV20CAV2010081.1CAV2081.1CAV2080.973.1CAV20
GUT88-8020AF152258CAV21CAV2110078.5CAV2178.5CAV2178.366.3CAV21
GUT88-8438AF152259CAV21CAV2110078.5CAV2178.5CAV2178.365.6CAV21
MD86-7277AF152265CAV21CAV2110093CAV2193CAV2192.967.2CAV21
WA89-9165AF152300CAV21CAV2110093.3CAV2193.3CAV2193.267.2CAV21
DOR93-1657AF081603CAV24CAV2410088CAV2488.2CAV2488.167.4CAV24
2106NE98AF252189CAV4CAV410085.7CAV485.7CAV485.165CAV4
1734O99AF290899CAV4CAV410086.6CAV486.7CAV486.166.2CAV4
727F99AF290903CAV6CAV610084CAV684.3CAV683.667CAV6
R15797AF252183CAV9CAV910085.1CAV985.1CAV984.170.2CAV9
1712NE99AF290898CAV9CAV910082.3CAV982.5CAV981.368.6CAV9
R7596AF252169CBV1CBV19479.9CBV180.1CBV17971.7CBV1
R6696AF252170CBV1CBV19479.7CBV180.3CBV178.770.9CBV1
HON84-6016AF152260CBV2CBV210086.2CBV286.2CBV285.269.8CBV2
MD84-5914AF152263CBV2CBV210087.4CBV287.4CBV286.568CBV2
NH97-2342AF081622CBV3CBV310080.8CBV380.8CBV379.469.6CBV3
BRA98-9169AF152249CBV3CBV310084.8CBV385CBV38470.1CBV3
BRA98-9171AF152250CBV3CBV310082.9CBV382.9CBV381.769CBV3
BRA88-9172AF152251CBV3CBV310084.8CBV385CBV38470.1CBV3
BRA88-9173AF152252CBV3CBV310085.1CBV385.3CBV384.370.4CBV3
PER89-9426AF152291CBV3CBV310081.8CBV381.6CBV380.470.4CBV3
R8797AF252182CBV4CBV410086.2CBV486.2CBV485.268.3CBV4
MEX88-8931AF152270CBV5CBV510087.2CBV587.2CBV586.370.3CBV5
PA88-8885AF152287CBV5CBV510085.1CBV585CBV58470.3CBV5
R3597AF252177CBV5CBV510093.1CBV593.1CBV592.670.9CBV5
R8697AF252178CBV5CBV510081.5CBV561.5CBV580.267.3CBV5
R9497AF252179CBV5CBV510093.1CBV579.3CBV577.967.8CBV5
1800NE99AF290900CBV5CBV510092.4CBV592.4CBV591.970.4CBV5
CB6IS2AF225470CBV6CBV69680.2CBV680.2CBV678.672.6CBV6
GA92-1616AF081606EV11EV119981.6EV1182.2EV1181.172.3EV11
WA92-1516AF081642EV11EV119980.5EV1181.1EV1179.973.7EV11
834NE99AF290904EV11EV119983.4EV1183.6EV1182.672.5EV11
2257NE99AF290908EV11EV119985.7EV1183.8EV1184.972.1EV11
ELS88-8236AF152256EV12EV1210082.2EV1282.1EV128171.2EV12
GV34AF252184EV12EV1210080.7EV1280.9EV1279.571.8EV12
M250AF252185EV12EV1210077.9EV1277.9EV1276.471.4EV12
VA86-6776AF152299EV13ND (ENV69-EV13)9674.2EV1374.4EV1372.7NTEV13
47-98AF290901EV14EV1410081.1EV1481EV1479.869.6EV14
CT92-1465AF081599EV16EV1610084.5EV1684.5EV1683.472.6EV16
875NE99AF290905EV17EV1710082.9EV1783.1EV178270.1EV17
1068NE99AF290907EV17EV1710084.1EV1784.3EV1783.271.3EV17
CT96-2182AF081601EV18EV1810078EV1878.2EV1876.870.2EV18
MD88-8208AF152269EV18EV1810079.1EV1879.3EV187870.2EV18
OK89-9448AF152282EV18EV1810078.9EV1879.1EV1877.870.2EV18
OR85-6323AF152284EV18EV1810080.3EV1880.2EV1878.970.2EV18
SC87-7477AF152292EV18EV1810082.1EV1882EV1880.970.2EV18
R100AF252187EV18EV1810076.4EV1876.9EV1875.468EV18
270N97AF252188EV18EV1810079.4EV1879.6EV1878.267.5EV18
M256AF290906EV2EV210077.9EV282.6EV281.571.4EV2
RI94-1959AF081633EV21EV2110079.8EV2179.8EV2178.570.8EV21
NC83-5515AF152275EV24EV2410078EV2478.1EV2476.769.9EV24
NC84-5530AF152276EV24EV2410078.2EV2478.3EV247769.6EV24
MD92-1649AF081615EV25EV2510078.5EV2578.9EV2577.569EV25
MN94-1828AF081618EV25EV2510078.3EV2578.7EV2577.368.7EV25
MO93-1808AF081619EV25EV2510078.9EV2579.4EV257868.3EV25
OR93-1817AF081627EV25EV2510079.1EV2579.6EV2578.269EV25
HON86-6843AF152261EV25EV2510080.9EV2580.9EV2579.769.8EV25
NC84-5531AF152277EV25EV2510079.4EV2579.6EV2578.270.6EV25
1498N98AF252181EV25EV2510081.8EV2581.8EV2580.668.9EV25
1573NE99AF290909EV25EV2510081.8EV2581.8EV2580.369.1EV25
T185AF290896EV29EV2910079.1EV2970.4EV681.170.1EV29
MD88-8157AF152266EV3EV310077.1EV377.5EV37669.1EV3
MT87-7421AF152274EV3EV310076EV378.3EV37769.1EV3
1431N98AF252171EV30EV3010087.8EV3088.5EV3087.169.9EV30
1432N98AF252172EV30EV3010087.8EV3088.5EV3087.769.9EV30
HC56AF252173EV30EV3010086.7EV3087.3EV308669.9EV30
127N88AF252174EV30EV3010088.7EV3088.9EV3088.270.1EV30
PER98-2558AF081632EV33EV3310080.6EV3380.6EV3379.270.4EV33
PA88-8412AF152286EV4EV410083.3EV483.3EV482.175.6EV4
T99AF252180EV4EV410082.1EV482EV480.873EV4
CT96-2181AF081602EV5EV510087.4EV587.3EV586.572.5EV5
NM95-2070AF081625EV6EV610080.1EV680.5EV679.269.7EV6
1201C99AF290897EV6EV610081EV680.4EV680.271.7EV6
1351N98AF252186EV7EV710082.3EV782.2EV781.169.8EV7
AR95-2139AF081596EV9EV910083.3EV983.3EV982.269.2EV9
NC92-1612AF081620EV9EV910084.9EV984.8EV983.869.9EV9
WI95-2151AF081645EV9EV910084EV983.9EV978.269EV9
SE74AF252166EV9EV910083.7EV983.9EV982.368.5EV9
MP211AF252167EV9EV910083.3EV983.5EV982.468.8EV9
T94AF252168EV9EV910082.6EV982.8EV981.768.5EV9
T22AF252175EV9EV910086.4EV986.4EV985.569.4EV9
R1192AF252176EV9EV910084.2EV984.2EV983.169.7EV9
M8/72D17604ENV70ENV7010098.7ENV7096.7ENV7098.668.9ENV70
V1250/81D17611ENV70ENV7010093.2ENV7093.4ENV7093.169.5ENV70
AL88-8149AF152248ENV71ENV7110099.1ENV7199ENV719970.7ENV71
MD87-9256AF152267ENV71ENV7110098.6ENV7198.6ENV7198.570.2ENV71
NM90-9873AF152278ENV71ENV7110083.6ENV7183.6ENV718366ENV71
OK89-9243AF152279ENV71ENV7110084.4ENV7184.2ENV7183.766.2ENV71
OK89-9452AF152283ENV71ENV7110083.4ENV7183.3ENV7182.765.9ENV71
TX89-9166AF152297ENV71ENV7110083.6ENV7183.6ENV718365.8ENV71
T100AF252190ENV71ENV7110084ENV7184ENV7183.466.7ENV71
OK85-6388AF152280NTUNT-GenB10072.3CBV670.8EV167.567.5EV4
VA86-6765AF152298NTUNT-GenB10073.5ENV6972.1EV170.368.8EV1
CT87-7122AF152254NTUNT-GenB10074.1CBV669.7EV867.767.7EV3
CT87-7123AF152255NTUNT-GenB10074.1CBV669.7EV867.567.3EV4
1827N98AF252165PV1PV1100100PV1100PV110071.2PV1
  • a Abbreviations: CAV, coxsackie A virus; CBV, coxsackie B virus; PV, poliovirus; NA, isolate not available for neutralization test. EV was directly detected and sequenced from the clinical sample; ND, not identified (bootstrap value, <65%); UNT, no reference strain was grouped into same cluster; Gen B, strain located in Cluster B of EV whole genome classification. NT, untypeable enterovirus (19).

  • b The field clinical samples added in this work are in boldface.

  • c The neutralization serotypes of the 64 sequences obtained from GenBank are as in the corresponding publications (17, 19).

  • d Highest value obtained after the application of equation 1 for the chosen alignment.

  • e Results that differing from the neutralization serotype are in boldface.

  • f Highest value obtained by the NW-GCG similarity test with GOP equal to 50 and GEP equal to 3. Values <75% are shaded in boldface since they do not meet the criterion for identification.

  • g Values higher than 70% are in boldface since they do not achieve the criterion for identification. Values higher than 75% are shaded in boldface and italic.