TABLE 1.

Comparison of physical status of HPV-16 DNA in invasive cervical cancers analyzed by four different molecular methods

Sample no.E2/E6 ratioaResult of:
Real-time PCRSouthern hybridization and multiplex PCR
10.53MixedbEpisomal
21.00EpisomalEpisomal
30.41MixedbMixed
41.11EpisomalEpisomal
50.91EpisomalEpisomal
60.94EpisomalEpisomal
70.55MixedbEpisomal
80.77EpisomalEpisomal
90.61EpisomalEpisomal
100.51MixedbMixed
110.87EpisomalEpisomal
121.13EpisomalEpisomal
  • a Obtained by comparison of copy numbers of genes.

  • b Concomitant form of HPV-16 DNA was confirmed by two-dimensional gel electrophoresis.