TABLE 1.

PCR and DNA sequencing oligonucleotides used for detection and analysis of LPVa

LPV regulatory region oligo- nucleotide pairSequenceReference positions (nt)bSize (bp) of PCR product (pLPV-K38)
LPR15′-GCAACTAGAC- CGCAGAACAGTTG-3′590-567520
LPR25′-CTCAGGGCAGC- TTACCTAATGAG-3′70-92
LPR35′-GGATAGCATCA- AGTGTAAATCCAG-3′790-767819
LPR45′-CTCTCCTCCTT- AGACAGCGTTTGG-3′5242-5265
  • a The temperature used was 65°C.

  • b Reference nucleotide positions in LPV reference strain K38.