TABLE 1.

Real-time PCR data for the resistant strains selected from our collection and sequencing data obtained for all mutant strains

Isolate no.Gene and codon no. affectedNucleotide substitutionAmino acid substitutionDeviation of Tm (°C) from that for wta
RPO1 probe (65.2°C)RPO2 probe (65.1°C)KATG probe (69.9°C)
1 rpoB 531TCG→TTGSer→Leu−6.41
katG 315AGC→ACCSer→Thr+2.70
2 rpoB 526CAC→TACHis→Tyr−1.42
katG 315AGC→ACCSer→Thr+2.80
3 rpoB 526CAC→CTCHis→Leu−0.66
4 rpoB 531TCG→TTGSer→Leu−6.41
katG 315AGC→ACASer→Thr+2.80
5 rpoB 531TCG→TGGSer→Trp−6.92
6 rpoB 526CAC→GACHis→Asp−3.03
7 rpoB 531TCG→TGGSer→Trp−6.76
8 rpoB 531TCG→TTGSer→Leu−6.52
9 rpoB 516GAC→GTCAsp→Val−2.48
katG 315AGC→ACCSer→Thr+2.80
10 rpoB 526CAC→GACHis→Asp−3.19
11 rpoB 515-516Deletion TG-GAsp-Asn→Met−8.39
12-18 katG 315AGC→ACC/ACASer→Thr+2.80
  • a Deviations in the Tms for the probes (RPO1, RPO2, and KATG) compared with the reference value for the wt (given in parentheses).