TABLE 1

RT-qPCR LLODs of HRV RNA transcripts by genotype sequence group and number of Con mismatches

Genotype sequence groupLLODa (copies/reaction)No. of Con mismatches
GTSCon
A10100
B10500
C50100
D50100
E1001001
F505001
G10102
H10101
I10010001
J100102
K50501
L50501
M100501
N100100001
R505001
U101002
  • a LLOD, lower limit of detection; GTS, genotype-specific primer/probe set; Con, consensus primer/probe set.