TABLE 1

The lower limit of detection of MeVA RT-qPCR compared to MeV RT-qPCR was determined by testing serial dilutions of synthetic MeV RNA with a known copy number

AssayCopy no.No. of samples with positive results/total no. of samples tested% positive results
MeVA RT-qPCR10318/18100
10218/18100
10113/2065
1001/186
10−1a0/30
MeV RT-qPCR10318/18100
10218/18100
10118/18100
1004/1822
10−1a0/30
  • a This concentration was tested only 3 times since it is undetectable by both assays and therefore was not informative in the determination of the lower limit of detection.