Table 3.

Reactivity of rabbit antisera in EIA withAcinetobacter LPS

Serum sample no.ImmunogenaTiter obtained with LPS from the following strainb:
34574424710861659ATCC 1790690ATCC 1117196
K32034 1,024,000 <1,000<1,0002,000<1,000512,000<1,00016,000<1,00016,0002,000<1,000<1,000
K32257<1,000 128,000 <1,000<1,000<1,000<1,00064,0001,000<1,00032,000<1,000<1,00016,000
K324444,000<1,000 64,000 <1,0001,000<1,000<1,0004,000<1,0001,0002,000<1,000<1,000
K3252464,000<1,0002,000 256,000 <1,000128,000<1,0004,000<1,00016,0002,0001,000<1,000
K32771,000<1,000<1,000<1,000 128,000 <1,000<1,000<1,000<1,0004,0001,0001,000<1,000
K33010816,000<1,000<1,0001,0002,000 1,024,000 1,00016,0004,0004,0004,0002,0002,000
K346611,000512,000<1,0002,000<1,0001,000 1,024,000 2,000<1,00032,0008,00016,00016,000
K34965<1,000<1,0001,000<1,000<1,000<1,0001,000 4,096,000 <1,0002,0001,000<1,000<1,000
K35198,000<1,000<1,00032,0001,000<1,000<1,0004,000 4,096,000 4,000<1,0001,000<1,000
K353ATCC 179064,0004,000<1,00016,000<1,0002,000<1,0002,000<1,000 4,096,000 <1,0002,0004,000
K35490<1,000<1,000<1,000<1,000<1,0008,000<1,0008,000<1,0008,000 2,048,000 8,0008,000
K355ATCC 11171<1,000<1,000<1,000<1,000<1,0008,000<1,000<1,000<1,0001,0002,000 2,048,000 2,000
K388962,0002,000<1,000<1,000<1,0004,000<1,0004,000<1,0004,0008,000<1,000 1,024,000
  • a Rabbits were immunized with heat-killed bacteria (see Materials and Methods).

  • b Highest antibody dilution yielding an optical density at 405 nm of >0.2 with 250 ng of antigen per well; homologous titers are printed in boldface.