Table 1.

Evaluation of the different experimental parameters as applied in the different participating laboratories

Center identificationElectrophoresis supplyGels per yearImaging softwareRun time (h)Voltage (V/cm)Pulsing protocolTemperature (°C)Gel percentage
1CHEF DR-II200Bio Image Advanced Q. 2.0120620 h: 5.3–34.9"141.0
2CHEF DR-II40–50GelCompar 3.125610 h: 5.0–15.0"; 15 h: 15.0–45.0"141.0
3CHEF DR-III35In-house software2667 h: 5.0–15.0"; 19 h: 15–60"141.0
4CHEF DR-II500GelCompar 4.048648 h: 1.0–80.0"121.2
5CHEF DR-II1000Taxotron20610 h: 10.0"; 10 h: 25.0"100.8
6Pulsaphor TM15In-house software27235c 4 h: 5.0"; 6 h: 15.0"; 8 h: 25.0"; 9 h: 35.0"121.2
7Gene Navigator100Taxotron24724 h: 20–80"101.0
8CHEF Mapper40GelCompar 4.020610 h: 5.0–15.0"; 10 h: 15.0–45.0"141.0
9CHEF DR-II150–200Molecular Analyst20620 h: 1.0–40.0"121.0
10CHEF DR-II100Molecular Analyst22622 h: 1.0–34.0"140.8
11CHEF DR-III200Biogene 6.322162 h: 0.5–5.5"; 18 h: 7.5 to 27.5"; 1 h: 50–65"141.0
12CHEF DR-II150Molecular Analyst2063 h: 1–10"; 17 h: 5–40"131.4
13a OptionalNAb Optional20620 h: 5.3–34.9"141
  • a The parameters for center 13 show the settings for the standardized protocol. Exact size of the gel not reported.

  • b NA, not applicable.

  • c The exact size of the gel was not reported, so the result is given as overall voltage.