Table 3.

Evaluation of the experimental data obtained by PFGE of MRSA: survey of molecular size valuesa

MRSA strainCenter 1Center 2Center 3Center 4Center 5Center 6Center 7Center 8Center 10Center 11Center 12Avg valueAvg value
IHGPIHGPIHGPIHIHIHIHGPIHGPIHGPGPIHIH (SD)GP (SD)
12,8132,6792,8462,8292,7632,7882,8482,4162,8132,9242,4002,7922,9322,7502,5502,7022,7102,768 (130)2,697 (165)
22,8132,6792,8282,8122,7792,7872,8562,4162,8132,7872,3872,7922,9322,7002,5802,7252,7162,750 (120)2,700 (164)
32,8132,6792,7852,8122,7852,7832,8742,4162,8132,8192,4682,7922,8322,7002,5702,6652,7042,750 (122)2,687 (124)
42,8132,6792,8182,8812,7862,7842,8542,4162,8132,7632,4672,7922,9322,7002,5502,6732,7192,747 (119)2,709 (156)
52,8132,6792,7962,8182,7702,7832,8812,4162,8132,9322,4942,7922,9322,7002,6102,6442,7202,763 (133)2,709 (135)
62,2272,0682,1532,3782,4522,1732,4811,9432,4022,2992,0762,2862,2652,0102,0202,2222,1332,239 (172)2,171 (118)
72,2472,0782,0902,3012,4602,1802,5401,7682,2432,2772,0452,2072,1792,0102,0502,0772,1582,200 (207)2,130 (87)
82,4122,2382,1292,3172,5942,3472,6731,9602,3782,4612,2262,4062,4602,2802,2202,1942,3002,359 (198)2,286 (88)
92,4002,2282,1112,3222,6112,3432,6812,0932,3832,4652,1892,4062,4492,3102,2202,7492,2832,374 (179)2,357 (180)
102,3702,2302,1042,3622,6162,3312,6561,9052,3832,4832,2062,3422,4352,2802,2302,1552,2562,340 (213)2,278 (92)
112,5932,6732,8112,7212,6752,6272,5672,6032,6903,4312,4562,5132,6772,7702,6802,4472,4762,713 (260)2,612 (104)
122,9122,7052,7012,7592,7872,7682,8541,8602,9312,6572,3582,9503,2992,7702,8302,7802,714 (317)2,786 (255)
132,5862,4832,6702,7012,5472,5412,4312,1252,7163,0232,4992,4032,5713,0802,5802,4642,2782,586 (287)2,548 (74)
142,8922,7522,9543,0522,7312,7532,7762,3712,8672,3602,9692,6742,8412,9002,6502,6942,7062,705 (193)2,816 (136)
152,6722,4702,7252,7902,5162,4912,5422,0802,5362,5402,5742,4642,5962,4002,6802,5022,5392,501 (166)2,586 (107)
162,7352,5442,8072,7262,1412,5872,7082,5312,9292,8212,3272,6312,7092,8102,6502,8052,7082,682 (208)2,628 (154)
172,5142,3072,7882,8332,4342,3962,7871,9402,6212,7702,1332,2962,7332,3302,4102,2822,1042,458 (279)2,442 (233)
183,9292,7942,7662,8172,4072,7092,4712,1922,7992,6452,4322,5722,7222,7102,8303,1072,6132,710 (441)2,773 (185)
191,9091,7372,4792,5932,5272,4412,6121,4021,8782,7261,4971,6561,8461,8001,8702,2632,5422,153 (448)2,035 (372)
202,4041,9252,6332,1332,7702,7852,8203,0772,6712,9661,9472,5772,3492,2102,8602,1172,681 (253)2,302 (354)
  • a The molecular length values are given in kilobase pairs. Centers 9 and 11 experienced some difficulties with efficient normalization and band recognition by the available software, hence, the absence of numerical data and the discrepancy of chromosome sizes for the identical isolates 1 to 5. For centers 4, 5, 6, and 11, the GP protocol did not give rise to interpretable results. Center 4 did not perform the GP analysis. SD, standard deviation. The average values are given per strain. When groups of isolates are considered, the following general averages were calculated: isolates 1 to 5, 2,756 (IH) and 2,700 (GP); isolates 6 to 10, 2,302 (IH) and 2,244 (GP); isolates 11 to 20, 2,590 (IH) and 2,552 (GP); IH, in-house protocol.