Table 1.

Serovars, stx genotypes, LEE-associated genes, and adhesive and hemolytic properties of bovine Stx-producingE. coli of serogroup O118 (n = 42)

Serovar and strainClinical status of calfstxgenehlyEHECHlyEHECHybridization with LEE probes A to DType of adhesion on:Intensity of FAS reactivity ona:
HEp-2 cellsFCL cellsHEp-2 cellsFCL cells
O118:H16
 GS1236-1b No diarrhea stx 1 +++LALA++++++
 GS1232-1No diarrhea stx 1 +++LALA+++
 GS1161-1b No diarrhea stx 1 +++LALA++++
 GS1180-1No diarrhea stx 1 +++LALA++++
 GS1205-1No diarrhea stx 1 +++LALA+++
 RW2303c Diarrhea stx 1 +++LALA+++++++
 RW2126c Diarrhea stx 1 +++LALA++++++
 RW2125d Diarrhea stx 1 +++LALA+++++
 RW2173Diarrhea stx 1 +++LALA++++
 RW0136Diarrhea stx 1 +++LALA++++++
 RW0203Diarrhea stx 1 +++LALA+++++
 GS1181-1Diarrhea stx 1 +++LALA++++
 AS2169Diarrhea stx 1 +++LALA++++
 340Diarrhea stx 1 +++LALA+++
 RW2199Diarrhea stx 1 +++LALA+++
 666/89Diarrhea stx 1 +++LALA++
 RW2022Diarrhea stx 1 +++LALA++
 RW2266Diarrhea stx 1 +++LALA+++
 RW1306e Diarrhea stx 1 +++LALA++
 RW1912Diarrhea stx 1 +++LALA+
 RW1362Diarrhea stx 1 ++NegativeLA+
 RW2121Diarrhea stx 1 NegativeLA
 RW1303f Diarrhea stx 1 +++NegativeLA+
 RW1366Diarrhea stx 1 +++NegativeLA++++
 RW1319Diarrhea stx 1 +++NegativeLA+++
 RW1305Diarrhea stx 1 +++NegativeLA+
 RW1817Diarrhea stx 1 +++NegativeLA+
 RW1307e Diarrhea stx 1 +g ++NegativeLA+
 RW1318Diarrhea stx 1 +++NegativeNegative+++
 RW1299Diarrhea stx 1 +++NegativeNegative+
 RW1304f Diarrhea stx 1 +++NegativeNegative+
 RW2017Diarrhea stx 1 +++NegativeNegative
 RW1365Diarrhea stx 1 ++NegativeNegative++
 RW2116Diarrhea stx 2 +++LALA+++
 RW2039Diarrhea stx 2 +++LALA+
 RW2136Diarrhea stx 2 +++LALA+
 RW2297d Diarrhea stx 1/2 +++LALA+++++
 RW1911Diarrhea stx 1/2 +++LALA++
O118:H−
 557/89Diarrhea stx 1 +++LALA++
 RW2311Diarrhea stx 1 +++LALA
 RW1302Diarrhea stx 1 +++NegativeLA+++
 RW2030Diarrhea stx 2 +++LALA++
  • a Numbers of infected cells with actin accumulation. −, FAS negative; +, ≤1% of infected cells positive; ++, >1 to ≤10%; +++, >10 to ≤50%; and ++++, >50 to ≤100%.

  • b Strains GS1236-1 and GS1161-1 were isolated from the same animal.

  • c Strains RW2303 and RW2126 were isolated from the same animal.

  • d Strains RW2125 and RW2297 were isolated from the same animal.

  • e Strains RW1306 and RW1307 were isolated from the same animal.

  • f Strains RW1303 and RW1304 were isolated from the same animal.

  • g Strain RW1307 reacted negatively with PCR probe Ehly1-5 but positively with DNA probe Ehly1-Ehly5.