Table 2.

Evaluation of the experimental data obtained by PFGE of MRSA: survey of visual data interpretationa

MRSA strainCenter 1Center 2Center 3Center 4Center 5Center 6Center 7Center 8Center 9Center 10Center 11Center 12
IHGPIHGPIHGPIHGPIHGPIHGPIHGPIHGPIHGPIHGPIHGPIHGP
1AAAAAAAAAAAAAAAAAAAA
2AAAAAAAAAAAAAAAAAAAA
3AAAAAAAAAAAAAAAAAAAA
4AAAAAAAAAAAAAAAAAAAA
5AAAAAAAAAAAAAAAAAAAA
6BBBBBBBBBBBBBBBBBBBB
7B1B1B1B1B1 C B1B1B1B1B1B1B1B1B1B1B1B1B1B1
8B2B2B2B2B2 D B2B2B2B2B2B2B2B2B2CCB2B2B2
9B3B3B3B3B3 C1 B3 B3B3 B3 B3 B3B3B3B3DDB3B2B3
10B4B4B4B4B4 C2 B3 B4B4 B3 B3 B4B4B4B4D1D1B4B3B4
11CCCCCECCCCCCCCCEECCC
12DDDDDFDDDDDDDDDFFDD
13EEEEEGEEEEEEEEEGGEEB3
14FFFFFHFFFFFFFFFHHFFD
15GGGGGIGGGGGGGGGIIGGE
16HHHHHJHHHHHHHHHJJHHB
17IIIIIKIIIIIIIIIKKIIF
18JJJJJLJJJJJJJJJLLJJG
19KKKKKMKKKKKKKKKMMKKG
20LLLLLNLLLLLLLLLNNLL
  • ↵a Observations differing from the experimental gold standard are highlighted in bold lettering. IH, in-house protocol. The following centers modified the commercial protocol as follows: center 1, other source for SmaI; center 4, use of own plugs and prolonged electrophoresis time; center 8, use of homemade plugs. For centers 4, 5, 6, and 12, no useful GP data could be generated.