Table 3.

Effect of specimen type on the performance of PCR for determination of acute C. pneumoniae infections in 156 patients with CAP requiring admission

Gold standardNo. (%) of patients positiveSpecimen evaluatedSensitivity (%)Specificity (%)NPV (%)PPV (%)
MIF assay15 (9.6)Throat20.089.991.217.6
Throat wash6.795.690.314.3
Nasopharynx40.089.993.330.0
Expanded gold standard 1a 39 (25.0)Throat43.6100.083.9100.0
Throat wash17.9100.077.9100.0
Nasopharynx51.3100.085.8100.0
Expanded gold standard 2b 12 (7.7)Throat16.789.492.711.8
Throat wash8.395.792.414.3
Nasopharynx33.388.794.020.0
Expanded gold standard 3c 23 (14.7)Throat17.490.086.123.5
Throat wash8.796.185.528.6
Nasopharynx36.490.989.640.0
  • a PCR and culture positive and/or MIF assay results indicating acute C. pneumoniae infection.

  • b MIF assay and rDNA LPS ELISA results indicating acute C. pneumoniae infection.

  • c MIF assay and/or rDNA LPS ELISA results indicating acute C. pneumoniae infection.