Table 2.

Base sequences and gene locations for the S. aureus exotoxin-specific 5′-biotinylated oligonucleotide probes

GeneaProbebOligonucleotide sequence (5′→3′)Nucleotide location within gene
sea SEA-7BGGA GTT GGA TCT TCA AGC AAG ACG531–554
seb SEB-3BGAG AAT AGC TTT TGG TAT GAC ATG901–924
sec c SEC-5BAAC GGC AAT ACT TTT TGG TAT GAT772–795
sed SED-5BTAA AGC CAA TGA AAA CAT TGA TTC A382–406
see SEE-5BCTT TGG CGG TAA GGT GCA AAG AGG C594–618
tst TST-5BAAG CCA ACA TAC TAG CGA AGG AAC348–371
eta ETA-5BCCA TGC AAA AGC AGA AGT TTC AGC414–437
etb ETB-5BTAC CAC CTA ATA CCC TAA TAA TCC AA190–215
  • a Sequences and locations were derived from the published nucleotide sequences for SEA (7), SEB (18), SEC1 to SEC3 (10,13), SED (2), SEE (14), TSST-1 (8), and ETA and ETB (20).

  • b 5′ biotinylated.

  • c The probe sequence used here covers the SEC1 to SEC3 subtypes.