Table 1.

Comparison of TUBEX with other immunoassays

SerumaResult with the following testb:
TUBEXInh-ELISAInh-slideIgM ELISAIgG ELISAAgg-wellAgg-slideWidal (TO)Widal (TH)
P180.04+1.101.20400400
P2a60.02+1.400.22+ND200400
P2b50.01ND1.300.88+ND200400
P340.060.420.84ND200400
P460.160.670.33++400200
P56ND+0.931.40200100
P660.01NDNDNDND50100
P770.020.411.10100100
P850.150.801.20400100
P930.14ND0.140.59+ND400100
P1060.060.900.85<50<50
P11a50.120.170.58<50<50
P11b70.01+0.221.30<50<50
P1250.010.901.0050100
P1350.090.900.57<5050
P1480.01+1.200.69++400400
P1580.08ND1.991.94ND+200100
P1670.08ND1.381.31ND100<50
P1770.13ND1.641.60ND+400200
P1860.20ND0.500.51ND50100
P1960.04ND0.410.72ND800<50
P208NDNDNDNDNDND400400
P217NDNDNDNDND400200
P2250.23ND0.330.39ND<501,600
P2350.27ND0.630.58ND<50200
P2450.13ND0.470.45ND<50200
P2570.04ND1.881.72ND+400800
N110.270.160.17<50<50
N220.240.290.62200<50
N300.40ND0.010.20NDND200<50
N400.23ND0.030.15ND100<50
N51NDND0.080.02ND50<50
N600.27ND0.130.11NDND<50<50
N700.28ND0.080.20ND<50<50
N800.270.040.10ND<50<50
N900.34ND0.030.04NDND50<50
N1000.24ND0.010.22ND<50<50
N1100.390.040.09100<50
N1200.28ND0.070.07NDND<50<50
N1300.27ND0.060.27NDND<50<50
N140NDNDNDNDND50<50
N1500.22ND0.030.04ND<50<50
N1600.410.090.05ND50<50
N1710.260.060.26ND50<50
N1820.290.040.09<50<50
N1900.15ND0.030.06NDND<50<50
N2000.24ND0.090.29NDND<50<50
N210ND0.050.36<50100
N2200.40ND0.020.04NDND<50<50
N2300.24ND0.080.035050
N2400.26ND0.020.01ND5050
N2500.41ND0.030.02NDND5050
N2600.310.080.19+5050
N2700.27ND0.010.075050
N2800.33ND0.060.085050
N2900.32ND0.030.2350800
N3000.310.010.0950<50
N3100.27ND0.050.10NDNDND
N3200.300.090.07NDND
N3300.39ND0.030.17NDNDND
N3400.36ND0.040.02NDNDND
N3500.30ND0.020.05NDNDND
N3600.33ND0.000.06NDNDND
N3700.310.010.09NDND
N3800.350.010.06NDND
N3900.33ND0.010.15NDNDND
N4000.37ND0.040.12NDNDND
N4100.36ND0.100.19NDNDND
N4200.29ND0.030.10NDNDND
N4300.330.040.06NDND
N4420.200.030.11NDND
N4500.24ND0.030.05NDNDND
N4600.27ND0.090.07NDNDND
N4700.32ND0.080.16NDNDND
N4800.33ND0.010.02NDNDND
N4900.27ND0.050.17NDNDND
N5000.380.080.08NDND
N5100.26ND0.040.04NDNDND
N5200.37ND0.060.13NDNDND
N5300.31ND0.120.04NDNDND
N5400.26ND0.060.14NDND
N5500.24ND0.080.04NDNDND
N5600.29ND0.040.25NDNDND
N5700.40ND0.010.11NDNDND
N5800.210.140.06+NDND
N5900.26ND0.070.32NDNDND
N6000.27ND0.030.17NDNDND
N6100.16ND0.090.12NDNDND
N6200.240.010.27NDND
N6300.210.050.42NDND
N6400.28ND0.060.16NDNDND
N6510.260.070.53NDND
  • a Sources of sera were as follows: P1 through P14, 14 patients with bacteriologically confirmed cases of typhoid; P15 through P25, 11 patients clinically diagnosed as having typhoid fever but not bacteriologically investigated; N1 through N26, 26 healthy blood donors; N27 through N65, patients suspected of having autoimmune disease. In addition, the TUBEX text was performed on six Weil-Felix- or R. mooseri-positive serum samples (N66 through N71; five with a score of 0 and one with a score of 1) and on seven samples from six septicemic patients (N72 through N78; six with a score of 0 and one with a score of 1).

  • b Inh-ELISA, inhibition ELISA; Inh-slide, inhibition slide agglutination test; Agg-well, well agglutination test; Agg-slide, direct slide agglutination test. Results are expressed as scores (ranging from 0 to 10) for the TUBEX test (see Materials and Methods), as ODs at 490 nm for all ELISAs, as reciprocals of titers for the Widal test, and as positive (+) or negative (−) reactions for the inhibition slide agglutination, direct slide agglutination, and well agglutination tests. ND, not done. Positive results are ≥3 for TUBEX, ≤0.2 for the inhibition ELISA, ≥0.14 for the IgM ELISA, ≥0.38 for the IgG ELISA, and ≥1:50 for the Widal test.