Table 3.

Results of testing reference strains for staphylococcal enterotoxin genotype derived from agarose gel analysis of multiplex PCR and colorimetric microtiter plate DNA EIA

Reference strainToxin phenotypeResult by:
Agarose gel analysisaColorimetric assay (OD450/630 ratio)
seasebsecsedseeseasebsecsedsee
KN543SEA+0.8530.0370.0620.0210.018
KN556SEA+0.7990.0360.0620.0190.019
KN560aSEA+1.0350.0630.0300.0300.018
H390SEA, SED++0.8670.0410.0630.8170.019
ATCC 14458SEB+0.0211.3640.1170.0500.022
62/92b SEB+0.0181.1690.0660.0540.036
H288aSEB+0.0281.5400.0370.1400.018
st 20783SEB+0.0160.9810.0550.0410.021
ATCC 19095SEC+0.0150.0431.3350.0210.025
1229/93b SEC+0.0150.0381.4060.0220.020
H494SEC+0.0210.0242.2000.0260.018
ATCC 23235SED+0.0130.0420.1231.2140.021
1634/93b SED+0.0190.0240.1281.4750.018
ATCC 27664SEE+0.0210.0340.0990.0201.819
Cowan 1c 0.0140.0830.0390.0190.017
ATCC 20044d 0.0200.0310.1100.0240.018
  • a −, negative; +, positive (judged by eye).

  • b Kindly provided by W. Witte (Robert Koch-Institut Wernigerode, Germany).

  • c S. aureus negative control strain.

  • d S. epidermidis negative control strain.