Table 1.

Base sequences, gene locations, and predicted sizes of PCR products for the S. aureus exotoxin-specific oligonucleotide primers

GeneaPrimerOligonucleotide sequence (5′→3′)Nucleotide location within geneSize of PCR product (bp)
sea SEA-3b CCT TTG GAA ACG GTT AAA ACG487–507127
SEA-4b TCT GAA CCT TCC CAT CAA AAA C592–613
seb SEB-1c TCG CAT CAA ACT GAC AAA CG634–653477
SEB-4b GCA GGT ACT CTA TAA GTG CCT GC1088–1110
secd SEC-3b CTC AAG AAC TAG ACA TAA AAG CTA GG665–690271
SEC-4b TCA AAA TCG GAT TAA CAT TAT CC913–935
sed SED-3b CTA GTT TGG TAA TAT CTC CTT TAA ACG354–380319
SED-4b TTA ATG CTA TAT CTT ATA GGG TAA ACA TC644–672
see SEE-3b CAG TAC CTA TAG ATA AAG TTA AAA CAA GC482–510178
SEE-2c TAA CTT ACC GTG GAC CCT TC640–659
tst TST-3AAG CCC TTT GTT GCT TGC G51–69445
TST-6ATC GAA CTT TGG CCC ATA CTT T474–495
eta ETA-3b CTA GTG CAT TTG TTA TTC AAG ACG374–397119
ETA-4b TGC ATT GAC ACC ATA GTA CTT ATT C468–492
etb ETB-3b ACG GCT ATA TAC ATT CAA TTC AAT G51–75262
ETB-4AAA GTT ATT CAT TTA ATG CAC TGT CTC286–312
  • a Sequences and locations were derived from the published nucleotide sequences for SEA (7), SEB (18), SEC1 to SEC3 (10,13), SED (2), SEE (14), TSST-1 (8), and ETA and ETB (20).

  • b Modified oligonucleotide primers based on primer sequences published by Johnson et al. (17).

  • c Oligonucleotide primer sequences as described by Johnson et al. (17).

  • d The primer sequences used here cover all three subtypes of SEC.