Table 5.

Detection of exotoxin-producing S. aureusreference strains (n = 23) and clinical isolates (n = 50) by immunoassays and multiplex PCR-EIA

Toxin phenotypeNo. of strainsNo. of positive samples detected by:No. of differencesa
Immuno- assaysMultiplex PCR-EIA
SEA109101
 Reference strains4440
 Clinical isolates6561
SEB6660
 Reference strains444
 Clinical isolates222
SEC5550
 Reference strains333
 Clinical isolates222
SED4440
 Reference strains333
 Clinical isolates111
SEE1110
 Reference strains111
 Clinical isolates0
ETA4340
 Reference strains333
 Clinical isolates111
ETB2220
 Reference strains222
 Clinical isolates0
TSST-11212120
 Reference strains555
 Clinical isolates777
Double producerb 7770
 Reference strains222
 Clinical isolates555
Nonproducerc 39000
 Reference strains300
 Clinical isolates3600
  • a Differences between the test results from immunological and PCR methods.

  • b Double exotoxin producers: one reference strain with SEA and SED, one reference strain with ETA and ETB, four clinical strains with SEA and TSST-1, and one clinical strain with SEC and TSST-1, respectively.

  • c Nonproducer of staphylococcal exotoxins tested.