Table 3.

Isoelectric points and deduced amino acid sequence changes for β-lactamases from Klebsiella outbreak strains

OrganismpIPCR results for:Amino acid (codon)a,b at position:Enzymea
TEMSHV394269104153164165182205237238240244265275276
E. coli 5.4QAME (GAG)HR (CGT)WMQAG (GGT)ERTRNTEM-1c
Klebsiella D56.0 + 7.6+K (AAG)S (AGT)TEM-15
D5 transconjugant6.0
Klebsiella D15.5 + 7.6+K (AAG)S (AGT)TEM-26
D1 transconjugant5.5
E. coli 7.6QGMDRRWTR (CGG)AG (GGC)E (GAG)RLRNSHV-1c
Klebsiella D27.6S (AGC)E (GAA)SHV-2
D2 transconjugant7.6+
Klebsiella D47.6S (AGC)E (GAG)SHV-2
D4 transconjugant7.6+
Klebsiella D87.6S (AGC)E (GAG)SHV-2
D8 transconjugant7.6+
Klebsiella D97.6 + 5.4S (AGC)E (GAG)SHV-2
D9 transconjugant7.6+
Klebsiella D37.75L (CTG)S (AGC)K (AAA)SHV-4
D3 transconjugant7.75+
Klebsiella D67.6 + 8.2S (AGC)K (AAG)SHV-5
D6 transconjugant8.2+
Klebsiella D75.4 + 7.6 + 8.2+S (AGC)K (AAG)TEM-1 + SHV-5
D7 transconjugant5.4 + 8.2+
Klebsiella D107.6 + 8.2S (AGC)K (AAG)SHV-5
D10 transconjugant8.2+
  • a Sequence and enzyme data apply to theKlebsiella-transconjugant pair as a whole.

  • b Amino acid abbreviations: A, alanine; D, aspartic acid; E, glutamic acid; G, glycine; H, histidine; I, isoleucine; K, lysine; L, leucine; M, methionine; N, asparagine; P, proline; Q, glutamine; R, arginine; S, serine; T, threonine; V, valine; W, tryptophan. Nucleotide abbreviations: A, adenosine; C, cytosine; G, guanosine; T, thymidine. The consensus numbering scheme for group 2 (molecular class A β-lactamases) (1) is used.

  • c Data from Jacoby and Medeiros (15), Huletsky et al. (14), Knox (20), and Bush and Jacoby (8).