TABLE 3

Malassezia species and proposed inocula for antifungal susceptibility testing

Strain no.SpeciesHostNo. of strains testedReference strainOD600 for inoculuma
1M. sympodialisHuman24CBS 72220.1
2M. slooffiaeHuman10CBS 79560.05
3M. furfurHuman5CBS 18780.005
4M. globosaHuman3CBS 79660.2–1
5M. restrictaHuman2CBS 78770.2–1
6M. obtusaHuman2CBS 78760.2–1
7M. dermatisHuman1CBS 91690.1
8M. japonicaHuman1CBS 94320.2
9M. yamatoensisHuman1CBS 97250.02
10M. nanaAnimal1CBS 95570.1
11M. pachydermatisAnimal1CBS 18790.01
12M. cuniculiAnimal1CBS 117210.1
13M. capraeAnimal1CBS 104340.1
  • a OD600 values for broth microdilution testing, as determined by plating 10 μl of a 1:10 dilution to achieve inocula of 104 to 105 CFU/ml. For the susceptibility assay, 50 μl of inoculum was added to 50 μl of 2× concentrations of antifungals, to achieve final cell densities of 5.0 × 103 to 5.0 × 104 CFU/ml.