TABLE 1

Agreement between Sensititre YeastOne and CLSI BMD method for anidulafungin, micafungin, and caspofungin susceptibility testing of Aspergillus spp.a

Species (no. of isolates)AntifungalCLSI MEC median (range)bYO color endpoints median (range)Median (range) 2-fold differenceAgreement (%)
±1 2-fold dilution±2 2-fold dilutions
A. flavus (9)ANID0.008 (≤0.002 to 0.015)≤0.015 (≤0.015 to ≤0.015)NDcNDND
MCF0.008 (≤0.002 to 0.015)≤0.008 (≤0.008 to 0.06)−1 (−4 to 1)6789
CAS0.06 (0.06 to 0.125)≤0.008 (≤0.008 to 1)3 (−4 to 4)00
A. fumigatus (17)ANID0.008 (0.008 to 0.015) [8, 16]≤0.015 (≤0.015 to ≤0.015) [8, 8]NDNDND
MCF0.008 (0.004 to 0.015) [8, 16]≤0.008 (≤0.008 to 0.03) [2, 4]0 (−1 to 2)88100
CAS0.25 (0.125 to 0.25) [16, 16]0.03 (≤0.008 to 0.125) [8, 8]3 (1 to 5)2441
A. terreus (13)ANID0.004 (0.004 to 0.008)≤0.015 (≤0.015 to ≤0.015)NDNDND
MCF≤0.002 (≤0.002 to 0.004)≤0.008 (≤0.008 to 0.015)−2 (−3 to −2)077
CAS0.125 (0.06 to 0.125)0.015 (≤0.008 to 0.06)2 (1 to 4)2354
  • a The agreement between Sensititre YeastOne (first purple well after 20 h for A. flavus and 30 h for A. fumigatus and A. terreus with 104 CFU/ml initial inoculum) and the CLSI BMD method for anidulafungin (ANID), micafungin (MCF), and caspofungin (CAS) susceptibility testing of Aspergillus spp. was evaluated.

  • b Where applicable, the CLSI MECs and YO color endpoints of the two resistant isolates are indicated within brackets.

  • c ND, not determined because of off-scale endpoints.