Table 1.

Primers used for typing VT genes

Primer pairSequence (5′ to 3′)Gene specificityLocation within geneSize of amplified product (bp)Reference or source
VT1-a VT1-b GAA GAG TCC GTG GGA TTA CG
AGC GAT GCA GCT ATT AAT AA
VT1From nucleotide 860 of A-subunit-coding region to nucleotide 31 of B-subunit-coding region130Pollard et al. (30)
VT2-a VT2-b TTA ACC ACA CCC ACG GCA GT GCT CTG GAT GCA TCT CTG GT VT2, VT2vh-a, VT2vh-b,
VT2d-Ount, VT2d-OX3a
From nucleotide 188 to nucleotide 533 of A-subunit-coding region346Pollard et al. (30)
VT2-c VT2-d AAG AAG ATG TTT ATG GCG GT CAC GAA TCA GGT TAT GCC TC VT2, VT2vh-a, VT2vh-bFrom nucleotide 4 of B subunit region to nucleotide 21 of upstream region285Tyler et
al. (38)
VT2v-1 VT2v-2 CAT TCA GAG TAA AAG TGG CC GGG TGC CTC CCG GTG AGT TC VT2vh-a, VT2vh-b, VT2d-Ount, VT2d-OX3aFrom nucleotide 110 of B-subunit-coding region to nucleotide 225 of upstream region385Tyler et
al. (38)
VT2-e VT2-f AAT ACA TTA TGG GAA AGT AAT A TAA ACT GCA CTT CAG CAA AT VT2, VT2vh-a, VT2vh-b,
VT2d-Ount, VT2d-OX3a
From nucleotide 883 of A-subunit-coding region to nucleotide 259 of B-subunit-coding region348This study
VT2-cm VT2-f AAG AAG ATA TTT GTA GCG G TAA ACT GCA CTT CAG CAA AT VT2d-Ount, VT2d-OX3aFrom nucleotide 4 to nucleotide 259 of B subunit region256This study
VTe-a VTe-b CCT TAA CTA AAA GGA ATA TA CTG GTG GTG TAT GAT TAA TA VT2eFrom nucleotide −25 downstream to nucleotide 205 of A-subunit-coding region230Johnson
et al. (9)