Table 1.

Comparison of results obtained by nested PCR and standard techniques with samples from a patient with cryptococcal meningitis

SampleaDate of sample (day/mo/yr)Test resultb with India inkCSF antigen titerSerum antigen titerTest resultc by:
CultivationNested PCR
128/5/97+++1:2,0481:2,048++
210/6/971:2,0481:2,048
326/6/97+1:1281:512
411/7/971:1281:128
56/9/971:321:128
611/11/971:81:128
  • a Sample 1 is the sample obtained before the patient started therapy. Sample 2 was obtained during pharmacological therapy, and samples 3 to 6 were obtained after therapy.

  • b +++, more than four C. neoformans cells per field; +, only one yeast cell observed in 10 fields; −, no yeast cells observed.

  • c +, positive; −, negative.