Table 2.

Sizes of the restriction fragments used for RFLP analysis

Primers used to amplify fragmentRestriction enzymeSize(s) of fragment(s) in genes coding for B subunit (bp)
VT2VT2cVT2d
VT2vh-aVT2vh-bVT2d-OuntVT2d-OX3a
VT2-c, VT2-da HaeIII285161, 124161, 124
RsaI216, 69136, 80 (69)b 216, 69
NciI285285159, 126
VT2-e, VT2-f HaeIII348216, 132216, 132216, 132167, 132 (49)b
PvuII323 (25)b 323 (25)b 250, 73 (25)b 200, 120 (28)b 200, 120 (28)b
  • a This primer pair was used according to the method of Tyler et al. (38).

  • b This fragment did not resolve or was too small to be clearly visible under the electrophoresis conditions used.