Table 3.

Biochemical characteristics of S. ficaria: comparison with published results

Test% Positivity
This study (n = 42 strains)Farmer et al. (5) (n = 13 strains)aGrimont et al. (9) (n = 14 strains)
Catalase100100
Cytochrome oxidase080
Nitrate reduction to nitrite6992100
Nitrate reduction to N231
H2S production000
Lipase0b77c93d
Gelatinase57100e100
DNase at 25°C43100100f
Urease000
Indole production000
Enzyme tests
 Arginine dihydrolase000
 Lysine decarboxylase000
 Ornithine decarboxylase000
 Tryptophan desaminase00g0g
Voges-Proskauer test717579
Esculin hydrolysis10010093
Oxidization of glucose100100
Acid from:
 Adonitol790100
l-Arabinose86100100
d-Arabitol9810079
 Cellobiose60100100
 Glucose100100100
 Inositol6255100
 Maltose86100100
 Mannitol95100100
 Melibiose554093
 Raffinose957086
 Rhamnose235100
 Sorbitol60100100
 Sucrose93100100
 Trehalose100100100
β-Galactosidase71100100
Utilization of:
 Citrate100h100i100i
 Malonate000
  • a Including 10 strains supplied by P. A. D. Grimont.

  • b Substrate, 5-bromo-indoxyl ester.

  • c Substrate, corn oil.

  • d Substrate, Tweens 40, 60, and 80.

  • e At 22°C.

  • f Temperature not indicated.

  • g Phenylalanine desaminase.

  • h A rate of positivity of 100% on Simmons citrate agar and also with Vitek GNI cards. This characteristic is very difficult to read when API 20E galleries are used (positive, 27%; negative; 19%; doubtful, 17%).

  • i Simmons citrate.