Table 5.

Long-term effect of storage at room temperature of unfixed cytospots on HCMV antigenemia assay sensitivity with the PV pool

Duration of storage at room temperatureNo. of replicatesMedian no. of pp65-positive PMN (range)P valueaMedian no. (%) of pp65-positive PMN with degree of staining
WeakModerateStrong
24 hours669.5 (58–76)15 (21)48 (69)6.5 (10)
10 days669 (58–81) NSb 15 (22)47 (68)7 (10)
NS
20 days867 (59–73)NSNS15 (22)45 (67)7 (11)
NS0.0055
30 days570 (53–91)NS0.000326 (37)38 (54)6 (9)
0.014 
40 days545 (43–47)0.001515 (34)27 (60)3 (7)
0.0002
55 days526 (20–28)0.000512 (46)12 (46)2 (8)
0.0001
75 days51 (0–5)0.00011 (100)0 (0)0 (0)
  • a t test.

  • b NS, not significant.