Table 4.

Effect of temperature during storage for a 7-day period on fixed and unfixed PMN slides with IFA and the PV pool

Storage temperatureFixation upon slide preparationNo. of replicatesMedian no. of pp65-positive PMN (range)P valuebMedian no. (%) of pp65-positive PMN with degree of stainingP valuec
WeakModerateStrong
RTa None5178.0 (160–196)30 (17)133 (75)15 (8)
Formalin + NP-405109 (86–117)0.000934 (31)70 (64)5 (5)0.012
0.0003 NSd
−80°CNone5129 (114–144)0.0040.02938 (29)87.5 (68)3.5 (3)0.004NS
0.0186       0.005
Formalin + NP-406157.5 (134–192)NS38.5 (24)99 (63)20 (13)NS
  • a RT, room temperature.

  • b t test.

  • c Pearson chi-square (P = 0.0016, overall comparison of three degrees of staining at different temperatures).

  • d NS, not significant.