Table 2.

Sensitivities of different immunostaining techniques with formalin plus NP-40 as fixative (prior to storage at −80°C) and the PV pool

Staining technique (No. of replicates)Median no. of pp65-positive PMN (range)P valueaMedian no. (%) of pp65-positive PMN with degree of stainingP valueb
WeakModerateStrong
IFA (10)205.5 (160–240)51.5 (25)130.5 (64)23.5 (11)
IPA (10)200 (145–230)NSc 33 (16)143 (72)24 (12)NS
] NS] NS
ABC (10)203 (148–245)NS36 (18)139.5 (69)27.5 (13)NS
  • a t test.

  • b Pearson chi-square test.

  • c NS, not significant.