Table 4.

Reactivities of recombinant MBG NP fragments to MBG antibody-positive samples determined by Western blotting and ELISA

SampleReactivitya/antigenic index of:
His-MBG-NPGST-MBG-GST
NP/C-halfNP5NP6NP7NP8
M1++/>64+/2−/8−/8+/>64−/1
M2++/>64−/2+/8−/2+/>64−/1
M3++/NTb +/NT+/NT−/NT+/NT−/1
M4++/>16−/2−/2−/2+/>16−/1
M5++/16+/4+/8−/2+/8−/1
M6++/>128+/64+/>128+/64+/>128−/1
M7++/16+/2−/2−/1+/8−/1
M8++/>128+/4+/32−/1+/>128−/1
 No. of WBcpositives8865180
 Average log2 antigenic indexd 5.42.03.31.75.10
  • a By Western blotting.

  • b NT, not tested.

  • c WB, Western blotting.

  • d In the calculation of log2antigenic indices of >16, >64, and >128, the values of the antigenic indices were regarded as 16, 64, and 128, respectively.