Table 4.

Genotypic characterization of enterobacterial isolates and their transformants, β-lactamase isoelectric points and plasmid DNA sizes of transformants, and phenotypic characterization of non-β-lactam antibiotic resistance markers

IsolatePCRaPCR (kb)aIsoelectric point(s)Plasmid size (kb)Associated non-β-lactam antibiotic resistance markersb
blaTEMblaSHVblaOXA-10arr-25′ CS-blaVEB-1blaVEB-1-3′ CSblaVEB-1-arr-2blaVEB-1-blaOXA-10
E. coli1+++Sp Ka Tb Ss Sx Cl Na Pe Ra
Transformant+++0.16.3; 0.80.737.4; 6.3; 5.4210Sp Ka Ss Cl Ra
E. coli2+++Sp Ka Tb Ss Sx Cl Te Na Pe Ra
Transformant++2.36.50.74.57.4; 6.3150Sp Ka Tb Ss Cl Ra
E. coli3+++Sp Ka Tb Ss Sx Cl Te Na Pe Ra
Transformant++2.38.2; 2.31.857.4; 6.3150Sp Ka Tb Ss Cl Ra
E. coli4+++Sp Ka Tb Ss Sx Cl Na Pe Ra
Transformant++4.26.50.737.4; 6.3150Sp Ka Tb Ss Cl Ra
E. coli5+++Sp Ka Tb Ss Sx Cl Te Na Pe Ra
Transformant++2.36.50.737.4; 6.3210Sp Ka Tb Ss Sx Cl Te Ra
E. coli6+++Sp Ka Tb Ss Sx Cl Ra
Transformant++2.36.50.737.4; 6.3150Sp Ka Tb Ss Cl Ra
E. coli7+++Sp Ka Tb Ss SX Cl Te Ra
Transformant++2.36.50.737.4; 6.3150Sp Ka Tb Ss Cl Ra
E. coli8+++Sp Ka Tb Ss Sx Cl Te Na Pe Ra
Transformant++2.38.657.4; 6.3150Sp Ka Tb Ss Te
E. coli9++Sp Ka Tb Ss Cl Te Ra
Transformant++2.36.50.737.4; 6.3150Sp Ka Tb Ss Cl Ra
E. coli10+++Sp Ka Tb Ss Sx Cl Te Ra
Transformant++2.36.50.737.4; 6.3210Sp Cl Ra
E. cloacae11+Ka Tb Ra
Transformant+1.33.97.4150Ka
E. cloacae12+Ka Tb Ra
Transformant+1.33.97.4150Ka
E. cloacae13++++Sp Ka Tb Ss Sx Cl Te Ra
Transformant++0.10.837.4; 6.3; 5.4100Ka Tb Ss Sx Ra
E. cloacae14++Sp Ka Tb Ss Sx Cl Ra
Transformant+0.10.87.4; 6.524Ss
E. sakazakii15+++Sp Ka Tb Ss Sx Cl Te Na Pe Ra
Transformant+++1.74.80.737.4; 6.3; 5.4150Ka Tb Te Ra
K. pneumoniae16+++Sp Ka Tb Ss Sx Ra
Transformant++2.36.50.737.4; 6.3150Sp Ka Tb Ss Ra
K. pneumoniae17+++Sp Ka Tb Ss Sx Cl Te Na Pe Ra
Transformant++1.44.80.737.4; 6.3210Sp Ka Tb Ss Sx Cl Te Ra
K. pneumoniae 18++++Sp Ka Tb Ss Sx Cl Te Ra
Transformant+++0.16.3; 0.80.737.4; 6.3; 5.4210Sp Ka Ss Cl Ra
  • a Minus (−) indicates that no PCR product was obtained.

  • b Antibiotic resistance markers: Sp, spectinomycin; Ka, kanamycin; Tb, tobramycin; Ss, sulfonamides; Sx, trimethroprim-sulfamethoxazole; Cl, chloramphenicol; Te, tetracycline; Na, nalidixic acid; Pe, pefloxacin; Ra, rifampin.