Table 1.

Oligonucleotide primers forCampylobacter MLST

LocusDideoxyoligonucleotide primerAmplicon size (bp)
FunctionName and sequence
ForwardReverse
asp Amplificationasp-A9, 5′-AGT ACT AAT GAT GCT TAT CC-3′asp-A10, 5′-ATT TCA TCA ATT TGT TCT TTG C-3′899
Sequencingasp-S3, 5′-CCA ACT GCA AGA TGC TGT ACC-3′asp-S6, 5′-TTA ATT TGC GGT AAT ACC ATC-3′
gln Amplificationgln-A1, 5′-TAG GAA CTT GGC ATC ATA TTA CC-3′gln-A2, 5′-TTG GAC GAG CTT CTA CTG GC-3′1,262
Sequencinggln-S3, 5′-CAT GCA ATC AAT GAA GAA AC-3′gln-S6, 5′-TTC CAT AAG CTC ATA TGA AC-3′
glt Amplificationglt-A1, 5′-GGG CTT GAC TTC TAC AGC TAC TTG-3′glt-A2, 5′-CCA AAT AAA GTT GTC TTG GAC GG-3′1,012
Sequencingglt-S1, 5′-GTG GCT ATC CTA TAG AGT GGC-3′glt-S6, 5′-CCA AAG CGC ACC AAT ACC TG-3′
gly Amplificationgly-A1, 5′-GAG TTA GAG CGT CAA TGT GAA GG-3′gly-A2, 5′-AAA CCT CTG GCA GTA AGG GC-3′816
Sequencinggly-S3, 5′-AGC TAA TCA AGG TGT TTA TGC GG-3′gly-S4, 5′-AGG TGA TTA TCC GTT CCA TCG C-3′
pgm Amplificationpgm-A7, 5′-TAC TAA TAA TAT CTT AGT AGG-3′pgm-A8, 5′-CAC AAC ATT TTT CAT TTC TTT TTC-3′1,150
Sequencingpgm-S5, 5-GGT TTT AGA TGT GGC TCA TG-3′pgm-S2, 3′-TCC AGA ATA GCG AAA TAA GG-3′
tkt Amplificationtkt-A3, 5′-GCA AAC TCA GGA CAC CCA GG-3′tkt-A6, 5′-AAA GCA TTG TTA ATG GCT GC-3′1,102
Sequencingtkt-S5, 5′-GCT TAG CAG ATA TTT TAA GTG-3′tkt-S4, 5′-ACT TCT TCA CCC AAA GGT GCG-3′
unc Amplificationunc-A7, 5′-ATG GAC TTA AGA ATA TTA TGG C-3′unc-A2, 5′-GCT AAG CGG AGA ATA AGG TGG-3′1,120
Sequencingunc-S5, 5′-TGT TGC AAT TGG TCA AAA GC-3′unc-S4, 5′-TGC CTC ATC TAA ATC ACT AGC-3′