Table 3.

Reactivities of the truncated NP fragments of EBO to EBO antibody-positive samples determined by Western blotting and ELISA

SampleReactivitya/antigenic index of:
His-EBO-NPGST-EBO-GST
NP/C-halfNP5NP6NP7NP8
E1++/16+/8−/4−/2+/8−/1
E2++/8−/1−/1+/4+/8−/1
E3++/4−/1+/4−/1+/8−/1
E4++/8−/1+/8−/2+/16−/1
E5++/64+/64+/32−/4+/32−/1
E6++/64+/8−/1−/8+/64−/1
E7++/NTb −/NT+/NT−/NT+/NT−/NT
E8++/>128+/32+/32+/32+/>128−/1
E9++/8+/1+/2−/1−/16−/1
E10++/>128+/8+/16−/8+/>128−/1
E11++/>128+/4+/16+/8+/>128−/1
E12++/>64+/2+/2−/2+/64−/1
E13++/>128+/16+/16−/16+/>128−/1
E14++/>128+/2+/8−/16+/>128−/1
E15++/32−/4+/4+/4+/64−/1
E16++/16+/2+/2−/2+/16−/1
E17++/8+/2+/1−/1+/2−/1
E18++/8+/8−/1−/2+/8−/1
E19++/32+/2+/16+/1+/2+/1
E20++/32+/4+/1−/1+/8−/1
E21++/>128+/8+/16−/8+/>128−/1
E22++/>128+/2−/2−/1+/64−/1
E23++/32−/4−/2−/2+/16−/1
E24++/>128+/>128+/>128+/64+/>128−/1
E25++/>128+/32+/64−/16+/>128−/1
E26++/>32+/>32+/16+/>32+/>32−/1
 No. of WBcpositives262620207251
 Avg log2 antigenic indexd 5.22.42.62.14.90
  • a By Western blotting.

  • b NT, not tested.

  • c WB, Western blotting.

  • d In the calculation of log2antigenic indices of >32, >64, and >128, the values of the antigenic indices were regarded as 32, 64, and 128, respectively.