TABLE 1

Capsular serotyping of C. canimorsus dog isolates by ELISA

Strain/isolateCapsular serovara
ABCDEFGHI
Cc5100 ± 020 ± 827 ± 1124 ± 617 ± 213 ± 414 ± 510 ± 413 ± 5
Cc5 Y1C1214 ± 6NDNDNDNDNDNDNDND
Cc632 ± 7100 ± 024 ± 1220 ± 413 ± 214 ± 612 ± 311 ± 414 ± 7
Cc6 ΔwbuBND14 ± 7NDNDNDNDNDNDND
Cc915 ± 317 ± 7100 ± 022 ± 417 ± 514 ± 513 ± 410 ± 314 ± 4
Cc9 ΔwbuBNDND20 ± 7NDNDNDNDNDND
Cc1219 ± 715 ± 523 ± 8100 ± 018 ± 014 ± 515 ± 510 ± 313 ± 5
Cc12 ΔwbtANDNDND20 ± 5NDNDNDNDND
Cc416 ± 314 ± 330 ± 1026 ± 5100 ± 013 ± 513 ± 410 ± 213 ± 5
CcD318 ± 711 ± 418 ± 519 ± 314 ± 610 ± 312 ± 115 ± 412 ± 6
CcD517 ± 413 ± 816 ± 422 ± 413 ± 611 ± 513 ± 214 ± 614 ± 6
CcD618 ± 1011 ± 520 ± 718 ± 315 ± 711 ± 314 ± 217 ± 416 ± 10
CcD1017 ± 812 ± 518 ± 317 ± 217 ± 510 ± 114 ± 215 ± 614 ± 6
CcD1316 ± 711 ± 521 ± 417 ± 212 ± 599 ± 112 ± 113 ± 412 ± 5
CcD1618 ± 710 ± 417 ± 486 ± 1411 ± 510 ± 313 ± 115 ± 512 ± 5
CcD1819 ± 912 ± 617 ± 515 ± 228 ± 1210 ± 215 ± 117 ± 613 ± 6
CcD2017 ± 611 ± 619 ± 317 ± 357 ± 2411 ± 312 ± 114 ± 412 ± 5
CcD2515 ± 611 ± 518 ± 417 ± 212 ± 59 ± 211 ± 114 ± 412 ± 3
CcD3320 ± 911 ± 617 ± 622 ± 213 ± 510 ± 312 ± 213 ± 4106 ± 30
CcD3416 ± 910 ± 416 ± 314 ± 213 ± 610 ± 212 ± 113 ± 413 ± 6
CcD3514 ± 712 ± 415 ± 512 ± 112 ± 411 ± 312 ± 214 ± 312 ± 5
CcD3715 ± 49 ± 319 ± 216 ± 012 ± 4100 ± 011 ± 112 ± 411 ± 5
CcD3914 ± 410 ± 518 ± 322 ± 214 ± 69 ± 211 ± 112 ± 312 ± 5
CcD4016 ± 811 ± 519 ± 419 ± 412 ± 510 ± 213 ± 216 ± 513 ± 6
CcD4320 ± 1024 ± 1486 ± 517 ± 113 ± 510 ± 312 ± 116 ± 114 ± 2
CcD4415 ± 89 ± 425 ± 716 ± 012 ± 58 ± 111 ± 112 ± 411 ± 5
CcD4716 ± 611 ± 617 ± 318 ± 012 ± 58 ± 212 ± 114 ± 311 ± 4
CcD5115 ± 714 ± 518 ± 420 ± 411 ± 59 ± 212 ± 014 ± 412 ± 5
CcD5216 ± 811 ± 716 ± 520 ± 611 ± 583 ± 413 ± 214 ± 412 ± 5
CcD5319 ± 812 ± 617 ± 218 ± 212 ± 59 ± 214 ± 241 ± 711 ± 4
CcD5717 ± 628 ± 1821 ± 423 ± 1232 ± 109 ± 213 ± 113 ± 412 ± 5
CcD5818 ± 711 ± 517 ± 322 ± 334 ± 1111 ± 214 ± 115 ± 513 ± 5
CcD6315 ± 911 ± 517 ± 117 ± 029 ± 1111 ± 5100 ± 014 ± 512 ± 5
CcD6843 ± 7110 ± 1116 ± 518 ± 013 ± 59 ± 213 ± 114 ± 511 ± 5
CcD6914 ± 711 ± 616 ± 313 ± 212 ± 58 ± 312 ± 112 ± 411 ± 5
CcD7115 ± 611 ± 517 ± 616 ± 213 ± 610 ± 213 ± 114 ± 513 ± 5
CcD7319 ± 712 ± 717 ± 023 ± 315 ± 615 ± 713 ± 518 ± 713 ± 5
CcD7613 ± 616 ± 814 ± 115 ± 315 ± 715 ± 713 ± 412 ± 413 ± 4
CcD7714 ± 913 ± 417 ± 714 ± 215 ± 714 ± 612 ± 510 ± 213 ± 4
CcD8016 ± 1113 ± 419 ± 517 ± 330 ± 913 ± 513 ± 510 ± 413 ± 5
CcD8116 ± 512 ± 423 ± 719 ± 422 ± 512 ± 512 ± 59 ± 311 ± 4
CcD8417 ± 814 ± 713 ± 118 ± 229 ± 1612 ± 512 ± 314 ± 413 ± 4
CcD8917 ± 615 ± 120 ± 695 ± 914 ± 914 ± 513 ± 413 ± 513 ± 5
CcD9620 ± 814 ± 119 ± 715 ± 1118 ± 3712 ± 411 ± 49 ± 313 ± 5
CcD10118 ± 512 ± 313 ± 416 ± 113 ± 712 ± 510 ± 3100 ± 012 ± 4
CcD10416 ± 914 ± 118 ± 721 ± 431 ± 1111 ± 410 ± 39 ± 311 ± 4
CcD105107 ± 2814 ± 216 ± 817 ± 514 ± 813 ± 511 ± 49 ± 312 ± 4
CcD10613 ± 816 ± 121 ± 1117 ± 259 ± 2413 ± 511 ± 49 ± 313 ± 5
CcD11315 ± 1116 ± 219 ± 914 ± 014 ± 6108 ± 611 ± 49 ± 412 ± 4
CcD11515 ± 916 ± 219 ± 921 ± 416 ± 812 ± 410 ± 49 ± 312 ± 5
CcD11614 ± 814 ± 219 ± 518 ± 215 ± 712 ± 512 ± 59 ± 313 ± 5
CcD11719 ± 1016 ± 119 ± 899 ± 1214 ± 713 ± 613 ± 59 ± 313 ± 5
CcD11815 ± 714 ± 120 ± 919 ± 415 ± 7111 ± 512 ± 410 ± 312 ± 5
CcD11915 ± 816 ± 216 ± 928 ± 2215 ± 912 ± 411 ± 412 ± 512 ± 4
CcD12015 ± 817 ± 317 ± 523 ± 716 ± 813 ± 512 ± 410 ± 312 ± 4
CcD12212 ± 814 ± 117 ± 613 ± 114 ± 813 ± 512 ± 49 ± 212 ± 4
CcD12415 ± 816 ± 119 ± 819 ± 415 ± 7109 ± 911 ± 49 ± 312 ± 4
CcD12613 ± 714 ± 116 ± 621 ± 315 ± 713 ± 611 ± 411 ± 412 ± 4
CcD12913 ± 915 ± 221 ± 620 ± 216 ± 812 ± 514 ± 610 ± 2100 ± 0
CcD13013 ± 729 ± 11108 ± 2619 ± 211 ± 615 ± 612 ± 410 ± 413 ± 5
CcD13112 ± 714 ± 217 ± 619 ± 314 ± 613 ± 611 ± 410 ± 312 ± 4
  • a Capsular serotyping was determined by ELISA on entire heat-killed bacteria. The following sera were used: Y1C12-adsorbed anti-Cc5 (A), Cc6 ΔwbuB adsorbed anti-Cc6 (B), Cc9 ΔwbuB adsorbed anti-Cc9 (C), Cc12 ΔwbtA adsorbed anti-Cc12 (D), anti-Cc4 adsorbed with all human isolates except Cc4 (E), anti CcD37 adsorbed with all human isolates (F), anti CcD63 adsorbed with all human isolates (G), anti CcD101 adsorbed with all human isolates (H), and anti CcD129 adsorbed with all human isolates (I). The readout of the ELISA was absorbance, but the results are expressed here as percentage of reactivity calculated with respect to the absorbance value obtained for the capsular type strain. Values are the mean ± standard deviation (SD) of the results from at least 3 independent experiments. Isolates with reactivities ≥30% appear in bold face. In addition, isolates with reactivities higher than 80% are highlighted in gray. ND, not determined.