Table 3.

PCR primers specific to R. salmoninarum DNA sequences that were used to amplify tRNA intergenic spacer regions

DesignationSequence (5′–3′)Sourcea
A35K+754TTGGTAAGAACGAGGTCACCGGATtRNA gene Thr (GGT)
A35K−760TTACCAAGAACGCGCTCTACCACTtRNA gene Thr (GGT)
T17C+80GACAGGATTCGAACCTGTGACCATtRNA gene Arg (TCT)
T17C−135TCAGTTGGATAGAGCATCCGCCTTtRNA gene Arg (TCT)
T25D+115CCTTAGTTCGTAGCCAAGTGCTCTtRNA gene Arg (ACG)
T25D−120TGGATAGAGCACTTGGCTACGAACtRNA gene Arg (ACG)
T35E+128ACCAACTGCGCTACAGGGCCTTGCtRNA gene Asp (GTC)
T35E−94GTTCAAGTCCCGTCAGGGTCGCTAtRNA gene Asp (GTC)
A7A+46GCGTGAAAGATCTTAACCGGTGAGtRNA ITS region
A7A−309ATGATCGACGTCAGCTCCATCAAGtRNA ITS region
A25A+4CCGGTGCTCTAACCAACTGAGCTAtRNA ITS region
A25A−247GGTTGTTGCGTTTAGCTCAAGACGtRNA ITS region
T3C+42GGCTCGTGTCAAGACGGTTTTTGAtRNA ITS region
T3C−123TGCTCTAACCAACTGAGCTACACCtRNA ITS region
T25E+19AACTGAGCTAAGCGCCCTTGAGAAtRNA ITS region
T25E−128CGCCATTTTCTAGATCCCTTGTGCtRNA ITS region
  • a tRNA gene anticodons are in parentheses.