Table 1.

Reaction buffers for the DNA polymerases

DNA polymeraseReaction buffer components
AmpliTaqGold10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl2, and 0.75 U AmpliTaq Gold DNA polymerase (Perkin-Elmer Cetus)
DyNAzyme II10 mM Tris-HCl (pH 8.8; 25°C), 1.5 mM MgCl2, 50 mM KCl, 0.1% TritonX-100, and 1 U of DyNAzyme II DNA polymerase (FINNZYMES OY, Riihitontuntie, Espoo, Finland)
DyNAzyme EXL50 mM Tris-HCl (pH 9.0; 25°C), 2.5 mM MgCl2, 15 mM (NH4)2SO4, 0.1% Triton X-100, and 1 U of DyNAzyme EXT DNA polymerase (FINNZYMES OY)
HotTub50 mM Tris-HCl (pH 9.0), 20 mM (NH4)2SO4, 2.5 mM MgCl2, and 0.75 U of HotTub DNA polymerase (Amersham Pharmacia Biotech)
Pwo10 mM Tris-HCl (pH 8.85; 20°C), 25 mM KCl, 5 mM (NH4)2SO4, 1.5 mM MgCl2, and 1.25 U of Pwo DNA polymerase (Roche Molecular Biochemicals)
rTth10 mM Tris-HCl (pH 8.3), 5% (vol/vol) glycerol, 0.1 M KCl, 0.05% (wt/vol) Tween 20, 0.75 mM EGTA, 2.5 mM MgCl2, and 1.25 U of rTth DNA polymerase (Perkin-Elmer Cetus)
Taq10 mM Tris-HCl, 1.5 mM MgCl2, 50 mM KCl (pH 8.3; 20°C), and 0.75 U ofTaq DNA polymerase (Roche Molecular Biochemicals)
Tfl20 mM Tris-acetate (pH 9.0), 10 mM (NH4)2SO4, 75 mM K-acetate, 0.05% Tween 20, 2.5 mM MgSO4, and 0.5 U of Tfl DNA polymerase (Promega Corporation, Madison, Wis.)
Tli10 mM Tris-HCl (pH 9.0; 25°C), 0.1% Triton X-100, 50 mM KCl, 2.5 mM MgCl2, and 0.3 U of TliDNA polymerase (Promega Corporation)
Ultma10 mM Tris-HCl (pH 8.8; room temperature), 10 mM KCl, 0.002% (vol/vol) Tween 20, 2.5 mM MgCl2, and 0.75 U of Ultma DNA polymerase (Perkin-Elmer Cetus)