TABLE 5

Broth media for Malassezia spp.

ComponentRPMI+aRPMI ++RPMI++ with esculin (OptiMAL)
MOPS (M)0.1650.1650.165
Sodium bicarbonate (mg/ml)0.20.20.2
Glucose (% of volume)222
Glycerol (% of volume)0.110.5
Glycerol monostearate (% of volume)0.05
Tween 20 (% of volume)0.04
Tween 60 (% of volume)0.50.5
Olive oil
Oleic acid (% of volume)22
Esculin (% of volume)0.1
Resazurin (μg/ml)12.512.512.5
pH6.326.256.26
  • a Adapted from Velegraki et al. (8).