Table 3.

In vitro susceptibilities of Candida species to four antifungal agents after 24 and 48 h of incubation

Species (no. of isolates)Antifungal agentMIC (μg/ml) at the indicated timea:% Resistant atb:
Range50%90%
24 h48 h 24 h48 h24 h48 h24 h48 h
C. albicans (240)Amphotericin B0.6–10.25–20.120.50.25100
Flucytosine≤0.06–4≤0.06–>64≤0.060.120.25103
Itraconazole≤0.008–>8≤0.008–>80.0160.030.03>8121
Fluconazole≤0.25–>256≤0.25–>256≤0.250.5≤0.25128113
C. glabrata (67)Amphotericin B0.12–10.5–40.2511407
Flucytosine≤0.06–4≤0.06–4≤0.06≤0.06≤0.060.1200
Itraconazole0.003–40.06–>80.251141358
Fluconazole1–>2562–>256486464913
C. parapsilosis (53)Amphotericin B0.03–0.50.06–20.120.50.25100
Flucytosine≤0.06–0.12≤0.06–1≤0.060.12≤0.060.2500
Itraconazole≤0.008–0.250.016–0.50.03250.120.2500
Fluconazole≤0.25–1≤0.25–40.511200
C. tropicalis (41)Amphotericin B0.06–10.25–20.2510.5100
Flucytosine≤0.06–>64≤0.06–>64≤0.060.250.1264510
Itraconazole≤0.008–4≤0.016–>80.060.250.5>8532
Fluconazole≤0.25–16≤0.25–>2560.518>256027
C. lusitaniae (15)Amphotericin B0.12–10.5–40.5114013
Flucytosine≤0.06≤0.06≤0.06≤0.06≤0.06≤0.0600
Itraconazole0.016–0.120.03–0.50.030.120.120.2500
Fluconazole≤0.25–32≤0.25–320.50.52700
C. krusei (15)Amphotericin B0.5–10.5–80.5214047
Flucytosine2–84–3281681607
Itraconazole0.12–10.25–20.250.50.51747
Fluconazole8–6416–1281664641281353c
  • a Inhibitory concentrations were determined spectrophotometrically by a microdilution method: ≥90% decrease in turbidity for amphotericin B and ≥50% decrease in turbidity for flucytosine, itraconazole, and fluconazole. 50% and 90%, MICs at which 50 and 90% of isolates were inhibited, respectively.

  • b Defined as follows: amphotericin B resistance, ≥2 μg/ml at 24 h and ≥4 μg/ml at 48 h; flucytosine resistance, ≥32 μg/ml; itraconazole resistance, ≥1.0 μg/ml; and fluconazole resistance, ≥ 64 μg/ml.

  • c Isolates of C. krusei were considered resistant to fluconazole irrespective of the MIC.