TABLE 1

List of plasmids used in assay development

Drug (gene)Nucleotide changeAmino acid change
PositionAmino acids
Rifampin (rpoB)CTG→CCGL511PLeucine→proline
CAA→CTAQ513LGlutamine→leucine
GAC→GTCD516VAspartic acid→valine
GAC→TACD516YAspartic acid→tyrosine
CAC→TGCH526CHistidine→cysteine
CAC→TACH526YHistidine→tyrosine
CAC→GGCH526GHistidine→glycine
CAC→CTCH526LHistidine→leucine
CAC→AACH526NHistidine→asparagine
CAC→CGCH526RHistidine→arginine
CAC→GACH526DHistidine→aspartic acid
CAC→CAGH526QHistidine→glutamine
TCG→TTGS531LSerine→leucine
CTG→CCGL533PLeucine→proline
WT
Isoniazid (katG)AGC→ACCS315TSerine →threonine
WT
Isoniazid (inhA promoter)−8 T→A
−8 T→G
−15 C→T
−16 A→G
−24 G→T
WT