Table 1.

The B. cepacia complex strain panel

Strain nameAccession no. from BCCM/LMG Culture CollectionSource and locationaStrain typebPresence of:Transformation ratecReference(s)
BCESMcblA
B. cepacia genomovar I
 ATCC 25416T LMG 1222T Onion, USA01102 9,22, 38
 ATCC 17759LMG 2161Soil, Trinidad15102 29
 CEP509LMG 18821CF, Australia18104 This study
 LMG 17997LMG 17997UTI, Sweden19102 This study
B. multivorans
 C5393LMG 18822CF, Canada03d Negligible 19
 LMG 13010T LMG 13010T CF, Belgium09105 24,30
 C1576LMG 16660CF-e, UK10104 30, 33
 CF-A1-1LMG 18825CF-e, UK11Negligible 23
 JTCLMG 18824CGD, USA12105 28
 C1962LMG 16665Clinic, UK24106 12
 ATCC 17616LMG 17588Soil, USA14103 3, 6, 29,30
 249-2LMG 18823Laboratory, USA14103 6
B. cepacia genomovar III
 J2315LMG 16656CF-e, UK02d ++Negligiblee 10,13
 BC7LMG 18826CF-e, Canada02d ++Negligiblee 26
 K56-2LMG 18863CF-e, Canada02d ++104 5,16
 C5424LMG 18827CF-e, Canada02d ++103e 19,20
 C6433LMG 18828CF-e, Canada04d +103e 19,20
 C1394LMG 16659CF-e, UK13d +103e 19,27
 PC184LMG 18829CF-e, USA17d +105e 17
 CEP511LMG 18830CF-e, Australia05+106 19
 J415LMG 16654CF, UK23105 8
 ATCC 17765LMG 18832UTI, UK06+105 29
B. cepacia genomovar IV
 LMG 14294LMG 14294CF, Belgium16d 102e 24
 C7322LMG 18870CF, Canada16d 103 19
 LMG 14086LMG 14086Respirator, UK16d 103 4
 LMG 18888LMG 18888Clinical, Belgium07103 31
B. vietnamiensis
 PC259LMG 18835CF, USA08d 103 2,15
 LMG 16232LMG 16232CF, Sweden20Negligible 30
 FC441LMG 18836CGD, Canada21104 This study
 LMG 10929T LMG 10929T Rice, Vietnam22106 7,30
  • a CF, infection of a CF patient; CF-e, strain that has spread epidemically among patients with CF; CGD, infection of a CGD patient; UK, United Kingdom; UTI, urinary tract infection.

  • b Strain types observed by RAPD and PFGE fingerprinting correlated for isolates examined in the panel; hence, each strain was given a single numerical strain type.

  • c Transformation rate per microgram of DNA electroporated into competent cells is the average number of Tpr colonies obtained from two independent experiments rounded up to the nearest log 10. Strains producing fewer than 100 Tpr colonies per electroporation were considered as having a negligible transformation rate.

  • d Strain type assigned for this study correlates to the numerical RAPD type assigned previously to this fingerprint pattern (19).

  • e Strains requiring selection on LB agar containing 400-μg/ml trimethoprim sulfate to overcome background due to intrinsic resistance; pUCP29T transformants from all other strains were selected on Luria-Bertani agar containing 100-μg/ml trimethoprim sulfate.