Table 1.

Characteristics of ELISAs for detection of T. pallidum antibodiesa

Trade name (company)Trepanostika (Organon)ICE Syphilis (Murex)Enzygnost Syphilis (Behring)Pathozyme Syphilis Competition (Omega)Bioelisa Syphilis (Biokit)Trep-Chek (Phoenix)TmpA-ELISA (Eurodiagnostica)Captia Syphilis G (Trinity)Captia Syphilis M (Mercia)19S IgM FTA-ABS
Predilution (μl; serum + diluent)NoNoNoNoYes (10 + 200)Yes (20 + 380)Yes (20 + 400)Yes (10 + 200)Yes (20 + 1,000)No
(Prediluted) serum (μl)30502525100100100210100
Diluent (μl)0500000000
Dilution1:11:21:11:11:211:201:211:211:511:10
Antigen T. pallidum lysateRec. TpN15 T. pallidum lysate T. pallidumlysateRec. TpN15Cocktail of Rec. antigensRec. TmpA T. pallidum    lysate T. pallidum lysateWhole treponemes
Rec. TpN17Rec. TpN17
Rec. TpN47
MethodCompetitiveCaptureCompetitiveCaptureSandwichSandwichSandwichSandwichCaptureImmunofluorescence
No. of incubations2322333332
Duration of all incubations (min)105120120115120751509015060
No. of washings1211222221
CutoffNC × 0.7NC + 0.200NC × 0.7Low positive controlNC + 0.300Cutoff controlNC + 0.100NC × 2Low positive control
Detection of IgIgM + IgGIgM + IgGIgM + IgGIgM + IgGIgM + IgGIgGIgM + IgGIgGIgMIgM
  • a Rec, recombinant; NC, negative control; ∗, overall dilution factor of IgM fraction of serum.