Table 2.

Predicted sizes of restriction fragments and enzymes used for restriction fragment length polymorphism analysis to validate the amplified products of multiplex PCR

GeneAmplicon size (bp)Multiplex primer setRestriction enzyme usedExpected sizes (bp) of restriction fragments
sea 102A AluI65, 37
seb 164A MaeIII120, 31, 13
sec 451A NdeI307, 144
sed 278A MboII175, 103
see 209A AccI119, 90
femA 132A or B AccI78, 54
eta 93B MboI57, 36
etb 226B HpaII159, 67
tst 326B MboI187, 139
mecA 163B MaeIII84, 79