Table 3.

Summary of results for acid stability, VDN test, pan-EV PCR, sequencing, and confirmatory neutralization test using monospecific antiseraa

IsolatebAcid stabilitycType by VDNdPCR/sequencing primer pairType by sequence% IdentityConfirmed by neutralizationeCategoryf
NC83-5515SE24012-011E2476.0Y1
NC84-5530SE24012-011E2476.5Y1
NC84-5531SE25012-011E2577.6Y1
TAI84-5839SCA16040-011CA1677.9TI1
MD84-5914LNT012-011CB286.2Y2
TX84-5915SE15187-222CB481.5Y2
HON84-6016SUNT040-011CB285.2Y2
MOR83-6264SUNT189-222CA1877.7Y3
MOR83-6266SUNT188-222CA2081.4Y3
MOR83-6282SUNT040-011CA2081.0Y3
MOR83-6286SE15187-222CB584.9Y2
OR85-6323SUNT012-011E1878.8Y2
OR85-6329SUNT187-222CB284.1Y2
OK85-6388SUNT012-011UNT EVE1: 68.0N4
MD86-6393SUNT187-222E384.4Y2
GA84-6536SUNT189-222CA2474.5TI3
IL85-6642SUNTEV2-006E481.3Y2
VA86-6765SUNT012-011UNT EVE1: 67.5N4
VA86-6776SUNT012-011E1372.3Y2
HON86-6843SE25040-011E2579.4Y1
CT87-7122SUNT012-011UNT EVE1: 66.5N4
CT87-7123SE15012-011UNT EVE4: 66.8N2
MD86-7277SUNT040-011CA2193.0Y3
MD86-7286SE33187-222CB597.2Y2
MT87-7421SUNT012-011E376.6Y2
SC87-7477SUNT012-011E1880.9Y2
GUT88-8020SUNT040-011CA2178.0Y3
AL88-8149SUNT040-011EV7181.6Y3
MD88-8157SUNT012-011E375.6Y2
OK88-8162LNT187-222UNT HRVHRV2: 73.4NT3
MD88-8208SUNT012-011E1878.1Y2
ELS88-8236SUNT012-011E1280.8Y2
TN88-8321SUNT040-011CA1779.1TI3
PA88-8412SUNT012-011E479.5Y2
GUT88-8438SUNT040-011CA2178.0Y3
PER88-8830SE11187-222E1180.0Y1
PER88-8831SE29187-222E2978.4Y1
PA88-8885SUNT012-011CB583.5Y2
MEX88-8931SCB5012-011CB586.0Y1
TX88-9121LNT187-222CB376.6Y3
WA89-9165SUNT040-011CA2193.3Y3
TX89-9166SUNT040-011EV7180.8Y3
BRA88-9169SE4040-011CB383.7TI3
BRA88-9171SE29012-011CB381.5Y3
BRA88-9172SE4012-011CB383.5Y3
BRA88-9173SE4040-011CB383.9Y3
OK89-9243SUNT040-011EV7181.1Y3
MD87-9256SUNT040-011EV7181.3Y3
PA89-9262SCA16040-011CA1677.7TI1
PER89-9426SNT012-011CB380.3Y3
OK89-9448SUNT012-011E1877.6Y2
OK89-9452SE5040-011EV7180.7Y3
NM90-9873SUNT040-011EV7180.3Y3
HON88-8429SE11187-222CB480.9Y2
CA90-0150LNT187-222HRV292.2NT3
  • a All isolates were successfully amplified with pan-EV RT-PCR primers.

  • b Isolates were named according to the state or country of origin and the year of original specimen collection and the laboratory identifier. Two-letter codes are standard state abbreviations. Three-letter codes are standard World Health Organization country codes (BRA, Brazil; ELS, El Salvador; GUT, Guatemala; HON, Honduras; MEX, Mexico; MOR, Morocco; PER, Peru; and TAI, Taiwan).

  • c S, acid stable; L, acid labile.

  • d UNT, untypeable by VDN; NT, not tested.

  • e Typed with monospecific antisera as described in Materials and Methods. Y, antigenic type agreed with molecular type; N, not neutralized by monospecific antisera against any of the four highest-scoring serotypes; TI, virus titer insufficient for antigenic typing; NT, not tested.

  • f As defined in Table 4.