Table 5.

Coamplification of gene targets

TemplateCTvaluea for the following target gene:
N. meningitidis38.00
H. influenzae31.37
S. pneumoniae26.40
N. meningitidis plusH. influenzae39.4431.69
N. meningitidis plus S. pneumoniae36.2927.07
H. influenzae plus S. pneumoniae31.3326.95
  • a CT value is the cycle number at which the measured fluorescent signal exceeds a calculated background threshold identifying amplification of the target sequence.