Table 1.

Sequences and position of oligonucleotide primers and probes

Gene targetSequence (5′ to 3′)
Forward primerReverse primerDye-labeled probea
ctrA617-GCTGCGGTAGGTGGTTCAA-635727-TTGTCGCGGATTTGCAACTA-7086-FAM–680-CATTGCCACGTGTCAGCTGCACAT-657
bexA142-GGCGAAATGGTGCTGGTAA-160241-GGCCAAGAGATACTCATAGAACGTT-217TET–189-CACCACTCATCAAACGAATGAGCGTGG-163
ply894-TGCAGAGCGTCCTTTGGTCTAT-915974-CTCTTACTCGTGGTTTCCAACTTGA-950VIC–941-TGGCGCCCATAAGCAACACTCGAA-918
  • a 6-FAM, 6-carboxyfluorescein; TET, tetrachloro-6-carboxyfluorescein.