Table 2.

Sequences of primers used for DNA amplification and size and localization of the cloned genes

GenesLocation and PCR primer sequencesaRestriction enzyme siteSize of DNA fragment (bp)Localization of DNA fragment (bp)bGenBank accession no.Reference
hsp705′ end, 5′-CATGccatggcGCGAAAAAAGAAAGTCTAACAAAAT-3′NcoI1,959151–2109M2758013
3′ end, 5′-CGggatccCTCAGGTTTATCAACAATTTCAACA-3′BamHI
hsp605′ end, 5′-CATGccatggTCGCTAAAAACATTAAATACAACGA-3′NcoI1,635383–2017M580278
3′ end, 5′-CATGagatctATAGTCCATTCCTGCGCCAGGCATTGC-3′BglII
pgp35′ end, 5′-CATGccatggGAAATTCTGGTTTTTATTTGTATAA-3′NcoI7954054–4848J0332112
3′ end, 5′-CATGagatctAGCGTTTGTTTGAGGTATTACCTCT-3′BglII
MIP5′ end, 5′-CATGtcatgaAGAATATATTAAGTTGGATGCTTAT-3′BspHId732100–831X66126 ,S47522 , X5348126
3′ end, 5′-CGggatccTTCTGTAACAGATACATTATCGTCG-3′BamHI
hsp105′ end, 5′-CATGccatggeCAGATCAAGCAACGACCCTCAAGAT-3′NcoI30937–345M580278
3′ end, 5′-CATGagatctTTGCAGAACTGCGATAACTTCGCTC-3′BgIII
  • a The lowercase characters correspond to restriction enzyme sites.

  • b Nucleotides are numbered according to coordinates of the sequences and refer to their position on the GenBank entries.

  • c G will be replaced by A with the QuickChange site-directed mutagenesis kit of Stratagene, La Jolla, Calif.

  • d The BspHI restriction site is TCATGA which is able to replace the NcoI (CCATGG), since CATG is conserved.

  • e G will be replaced by T with the Quick Change site-directed mutagenesis kit of Stratagene.