Table 4.

Specificities of N. meningitidis ctrA,H. influenzae bexA, and S. pneumoniae ply primers in multiplex and single PCR formats

Organism(s)No. testedNo. (%) reactive with the following primer set:
ctrAbexAply
N. meningitidis serogroup A33 (100)
N. meningitidis serogroup B2424 (100)
N. meningitidis serogroup C2525 (100)
N. meningitidis serogroup X33 (100)
N. meningitidis serogroup Y33 (100)
N. meningitidis serogroup Z33 (100)
N. meningitidis serogroup 29E33 (100)
N. meningitidis serogroup W13533 (100)
N. meningitidis, not groupable55 (100)
H. influenzae Pittman type b99 (100)
H. influenzae Pittman type c11 (100)
S. pneumoniae types 1, 2, 3, 4, 5, 6, 7, 8, 9, 10A, 11A, 12, 14, 15B, 17F, 18, 19, 20, 22, 23, 24, 31, and 333737 (100)
Othera 89
Total25372 (100)10 (100)37 (100)
  • a Other bacterial strains tested wereNeisseria lactamica (n = 5), N. gonorrhoeae(n = 4), N. sicca (n = 1), N. flavescens (n = 1), N. cinerea (n = 1),N. elongata (n = 1), N. pharyngis(n = 1), N. polysaccharidae (n = 1),Enterococcus faecalis (n = 5), Lancefield group B streptococci (n = 5), coagulase-negative staphylococci (n = 5), methicillin-resistant S. aureus(n = 5), E. coli (n = 5), E. coli K1 (n = 1), P. aeruginosa (n = 5), diphtheroids (n = 5), Proteus mirabilis(n = 5), Acinetobacter sp. (n = 5),K. pneumoniae (n = 5), Moraxella catarrhalis(n = 5), E. cloacae (n = 5), andH. influenzae types a (n = 1), d (n = 1), e (n = 1), and f (n = 1). Viruses tested were herpes simplex virus types 1 and 2 (n = 4), varicella-zoster virus (n = 1), cytomegalovirus (n = 2), and hepatitis B virus (n = 2).