Table 1.

Patients, clinical symptoms, origin of samples and genotype of different T. gondii strains characterized in this study

Patient group and designationaClinical symptomsbSampleNested PCR RFLP resultc
SAG2-3′SAG2-5′Type
Immunocompromised
  1 IHIV+NSCSFI
  2 IHIV+NSCSFI
  3 IHIV+GS, cachexiaBloodI
  4 IHIV−No dataBloodI
  5 IHIV+NSCSF+II
  6 IHIV+NSCSF+II
  7 IHIV+NSCSF+NAII
  8 IHIV+NSBrain (from biopsy)+II
  9 IHIV+NSCSF+II
 10 IHIV−NSBrain (from biopsy)+II
 11 IHIV−PS, feverLung (from biopsy)+II
 12 IHIV+Eye diseaseVitreous humor+II
 13 IHIV−Eye diseaseVitreous humor+II
 14 IHIV+NS, PS, retinochoroiditisCSF+III
 15 IHIV+NS2 brain samples (from biopsies)+III
 16 IHIV+NSBlood+III
 17 IHIV+NSCSF+III
 18 IHIV+NSCSFNAnon-III
 19 IHIV+NSCSFNAnon-III
 20 IHIV−NSCSFNAnon-II
Congenital infection
  1 CmaternalNo data on newbornAmniotic fluidI
  2 CmaternalNo data on newbornAmniotic fluidI
  3 CmaternalNo data on newbornAmniotic fluidI
  4 CmaternalNo data on newbornMaternal urineI
  5 CnewbornCC, VD, chorioretinitisBloodI
  6 CnewbornAbortion, cardiopathyAbortion ascitic fluidI
  7 CnewbornNewborn asymptomaticBlood+II
  8 CnewbornNewborn asymptomaticBlood+III
  9 CnewbornNo data on newbornBloodNAnon-II
 10 CnewbornNo data on newbornBloodNAnon-II
 11 CnewbornNewborn asymptomaticBloodNAnon-II
 12 CnewbornNewborn asymptomaticBloodNAnon-II
 13 CmaternalNo data on newbornMaternal bloodNAnon-II
  • a The patient designations indicate the HIV status and time of diagnosis of congenital infection as follows: IHIV+, immunocompromised HIV-positive patient; IHIV−, immunocompromised HIV-negative patient; Cmaternal, maternal diagnosis of congenital infection; Cnewborn, newborn diagnosis of congenital infection.

  • b Abbreviations; NS, neurological symptoms; GS, general symptoms; PS, pulmonary symptons; CC, cerebral calcifications; VD, ventricle dilation.

  • c SAG2-3′ andSAG2-5′, 3′- and 5′-end-amplified fragments ofSAG2; +, product amplified digested; −, product amplified not digested; NA, product not amplified; non-III, non-type III; non-II, non-type II.