Table 3.

Comparative end point sensitivities of primer sets in multiplex and single-set formats

TargetAssay formatCT valuea at the following dilution of DNA:
Undiluted10−110−210−310−4
N. meningitidis ctrA Multiplex26.9430.3932.9137.25>45.0
Single26.8530.0734.2538.73>45.0
H. influenzae bexA Multiplex28.5431.5834.62>45.0>45.0
Single28.4932.0737.96>45.0>45.0
S. pneumoniae ply Multiplex29.4132.2635.4036.32>45.0
Single29.7432.0635.2836.50>45.0
  • a The CT value is the cycle number at which the measured fluorescent signal exceeds a calculated background threshold identifying amplification of the target sequence; a value of >45.0 is deemed to be negative.