Table 1.

Sensitivity and intraexperimental variability of HHV-8 DNA quantification

Copy no.Control plasmid diluted in wateraControl plasmid diluted in human DNAb
Detection rate (%)MeanCT SD ofCT CVc(%)Detection rateMean CT SD ofCT CV (%)
106 10017.160.221.3
105 10020.660.301.5
104 10023.880.261.1
103 10027.480.210.7
102 10031.030.220.710031.910.210.6
101 10035.161.022.910035.761.193.3
100 7536.40d 0.83d 2.37537.76d 1.03d 2.7
  • a Measurements were repeated eight times in the same experiment.

  • b Measurements were repeated four times in the same experiment.

  • c CV, coefficient of variation.

  • d Calculated for samples withCT values of <45.