TABLE 3

Oligonucleotide sequences for CNS-TAC assaysa

TargetGeneIDSequence (5′–3′)Reference
CMVUL55ForAGG TCT TCA AGG AAC TCA GCA AGA26
RevCGG CAA TCG GTT TGT TGT AAA
PrFAM-ACC CCG TCA GCC ATT CTC TCG GC-BHQ 1
EBVBNRF1ForGGA ACC TGG TCA TCC TTT GC27
RevACG TGC ATG GAC CGG TTA AT
PrFAM-CGC AGG CAC TCG TAC TGC TCG CT-BHQ 1
Mycobacterium tuberculosisIS6110ForCCT ACT ACG ACC ACA TCAb
RevCCG TAA ACA CCG TAG TTG
PrFAM-ATG TGC TCC TTG AGT TCG CCA T-BHQ 1
Neisseria meningitidissodCForCCA CCC GTG TGG ATC ATA ATA GA28
RevGCA CAC TTA GGT GAT TTA CCT GCA T
PrFAM-CA TGA TGG CAC AGC AAC AAA TCC TGT TT-BHQ 1
Streptococcus pneumoniaelytAForACG CAA TCT AGC AGA TGA AGC A29
RevTCG TGC GTT TTA ATT CCA GCT
PrFAM-TG CCG AAA ACG CTT GAT ACA GGG AG-BHQ 1
VZVORF29ForCAC GTA TTT TCA GTC CTC TTC AAG TG30
RevTTAGACGTGGAGTTGACATCGTTT
PrFAM-TACCGCCCGTGGAGCGCG-BHQ 1
Chikungunya virus 1NSP1ForAAAGGGCAAACTCAGCTTCAC31
RevGCCTGGGCTCATCGTTATTC
PrFAM-CTGTGATACAGTGGTTTCGTGTG-BHQ 1
Chikungunya virus 2NSP4ForTCACTCCCTGTTGGACTTGATAGA31
RevTTGACGAACAGAGTTAGGAACATACC
PrFAM-AGGTACGCGCTTCAAGTTCGGCG-BHQ1
Enterovirus5′ UTRForGGC CCC TGA ATG CGG CTA ATC C32
RevGCG ATT GTC ACC ATWA GCA GYC A
PrFAM-CC GAC TAC TTT GGG WGT CCG TGT-BHQ1
Mycoplasma pneumoniaeCARDS toxinForTTT GGT AGC TGG TTA CGG GAA T33
RevGGT CGG CAC GAA TTT CAT ATA AG
PrFAM-TG TAC CAG AGC ACC CCA GAA GGG CT-BHQ1
HSV 1US4ForTAT TGG TGC GAT GGC GAC AC34
RevCTT TCC GCA TGT GGG CTC TC
PrFAM-CCC CGC CCC ATA CCC TAC CCG C-BHQ1
HSV 2US6ForAGC ATC CCG ATC ACT GTG TAC TA34
RevGCG ATG GTC AGG TTG TAC GT
PrFAM-CAG TGC TGG AAC GTG CCT GCC GC-BHQ 1
Measles virusNForTGG CAT CTG AAC TCG GTA TCA C35
RevTGT CCT CAG TAG TAT GCA TTG CAA
PrFAM-CCGAG GAT GCA AGG CTT GTT TCA GA-BHQ1
Mumps virusNPForGTA TGA CAG CGT ACG ACC AAC CT36
RevGCG ACC TTG CTG CTG GTA TT
PrFAM-CC GGG TCT GCT GAT CGG CGA T-BHQ 1
Parechovirus5′ UTRForGTAACASWWGCCTCTGGGSCCAAAAG37
RevGGCCCCWGRTCAGATCCAYAGT
PrFAM-CCTRYGGGTACCTYCWGGGCATCCTT-BHQ 1
BartonellassrAForGCTATGGTAATAAATGGACAATGAAATAA38
RevGCTTCTGTTGCCAGGTG
PrFAM-ACCCCGCTTAAACCTGCGACG-BHQ1
Haemophilus influenzaebexAForTGCGGTAGTGTTAGAAAATGGTATTATG39
RevGGACAAACATCACAAGCGGTTA
PrFAM-ACAAAGCGTATCAATACTACAACGAGACGCAAAAA-BHQ1
Acanthamoeba18S rRNAForCCCAGATCGTTTACCGTGAA40
RevTAAATATTAATGCCCCCAACTATCC
PrFAM-CTGCCACCGAATACATTAGCATGG-BHQ1
LyssavirusNForGTRCTCCARTTAGCRCACAT41
RevCACMGSNAAYTAYAARACNAA
PrFAM-CATCACACCTTGATGACAACTCACAA-BHQ1
Balamunthia mandrillaris18S rRNAForTAA CCT GCT AAA TAG TCA TGC CAA T40
RevCAA ACT TCC CTC GGC TAA TCA
PrFAM-AG TAC TTC TAC CAA TCC AAC CGC CA- BHQ1
Pan-dengue virus3′ NCRForGGA TAG ACC AGA GAT CCT GCT GT42
Rev 1CAT TCC ATT TTC TGG CGT TC
Rev 2CAA TCC ATC TTG CGG CGC TC
PrFAM CA GCA TCA TTC CAG GCA CAG-BHQ1
Nipah virusNForCTG GTC TCT GCA GTT ATC ACC ATC GA43
RevACG TAC TTA GCC CAT CTT CTA GTT TCA
PrFAM-CAG CTC CCG ACA CTG CCG AGG AT-BHQ
RNase PRPP30ForAGA TTT GGA CCT GCG AGC G44
RevGAG CGG CTG TCT CCA CAA GT
PrFAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ
  • a ID, oligonucleotide identity; For, forward; Rev, reverse; Pr, probe; CMV, cytomegalovirus; EBV, Epstein-Barr virus; VZV, varicella-zoster virus; HSV, herpes simplex virus; UTR, untranslated region; NCR, noncoding region; ORF, open reading frame.

  • b —, J. Posey, unpublished data.