Table 2.

Primers used to screen for interspecific variation in Malassezia species

Locus or regionPrimer setPrimer sequence (5′-3′)Product size (bp)Reference
Large-subunit rRNA  gene26S-Sa GCTGAACTTAAG 16
CATATCAT
26S-Ab TAGACGTTAGAC642 11
TCCTTGGT
ITSITS 1TCCGTAGGTGAA∼800/700 35
CCTGCGG
ITS 4TCCTCCGCTTATT
GATATGC
β-Tubulin geneBt-2aGGTAACCAAATC∼550 12
GGTGCTGCTTTC
Bt-2bACCCTCAGTGTA
GTGACCCTTGGC
Lipase genec LIP-FGTGTTGGCGTAC∼530 24
CCGTCGTT
LIP-RCGAGGTCGTTGG
CAAACGCA
  • a The primer designed correspond to nucleotides 1 to 20 of the partial sequence of M. furfur(CBS 1878; accession no. AF063214).

  • b The antisense primers for the LSU rRNA gene were designed from the sequence of Saccharomyces cerevisiae(accession no. J01355).

  • c Primers for the lipase region were designed from the sequence of the LIP2 gene of C. cylindracea (accession no. X64704). A 700-bp fragment that was conserved among the five accessioned sequences of lipase genes fromC. cylindracea was used for designing primers.